开放性颅脑损伤合并海水浸泡脑组织病理变化的实验研究

目的观察重型开放性颅脑损伤后海水浸泡对脑组织病理改变的影响。方法利用改进的兔重型开放性颅脑损伤模型进行30min海水浸泡,对照组不进行海水浸泡。分别在干预结束后0、3、8h在挫裂伤区取材,固定于10%福尔马林,作HE染色,光镜下观察创伤组织的病理改变。结果浸泡组和对照组在伤后先后出现了严重的创伤性脑水肿,但水肿高峰期出现时间不一致,对照组早在干预结束后3h即出现创伤性水肿改变,干预结束后8h水肿依旧;而海水浸泡组在干预结束后8h才出现明显的水肿,但水肿呈现进一步加重趋势。结论海水浸泡延迟了创伤性脑水肿高峰的出现,但发生的创伤性脑水肿重于单纯创伤。

Pathological changes in rabbit brain after traumatic head injuries with seawater immersion

Objective To observe the pathological impact of seaw at er on rabbit brain tissue with severe traumatic injury. Methods Modified rabbit models of severe brain injury was utilized, in which the damaged brain tissues were subjected to immersion with fresh seawater for 30 min. A control group was set up in which the trauma was induced without subsequent seawater immersion. Tissue sampling was performed at the brain injury sites at 0, 3, 8 h after seawa ter immersion and the pathological changes in the brain tissues were observed b y means of HE staining. Results Severe traumatic brain edema occurred in both o f the two groups, but the onset time of edema differed. In the control group, br ain edema was obvious at 3 h after treatment and hardly aggravated at hour 8; w hile in seawater treatment group, severe brain edema occurred at 8 h after the treatment and aggravated progressively. Conclusion Seawater immersion delays the onset and peak of traumatic brain edema following severe brain injuries, but c an eventually aggravate the traumatic edema.