黄荆子乙酸乙酯提取物对HL-60细胞生长和凋亡的影响

目的:观察黄荆子乙酸乙酯提取物(EVn-50)抑制人急性髓性白血病HL-60细胞生长和诱导凋亡作用。方法:体外培养HL-60细胞和人外周血单核细胞(PBMC),MTT法测定细胞增殖;软琼脂培养集落形成法检测细胞生长;PI染色流式细胞术分析细胞凋亡。结果:EVn-50对HL-60细胞增殖具有显著抑制作用,呈浓度依赖性;而对PBMC的增殖活性影响小。EVn-50对HL-60细胞集落形成有抑制效应,呈浓度依赖性。EVn-50有效诱导HL-60细胞凋亡。结论:EVn-50具有抑制HL-60细胞生长和诱导凋亡作用。

Effect of the Acetoacetate Extract of the Vitex Negundo Seed on the Growth and Apoptosis of Human Acute Myeloid Leukaemia HL-60 Cells

Objective To investigate the effect of the acetoacetate extract of the Vitex negundo seed(EVn-50) on the growth and apoptosis of human acute myeloid leukaemia HL-60 cells.Methods HL-60 cells and peripheral blood monouclear cells(PBMC) were cultured in vitro.The cell proliferation was detected by MTT assay.The cell growth was examined by colony formation method in soft agar.The cell apoptosis was analyzed using flow cytometry with PI staining.Results EVn-50 significantly inhibited proliferation of HL-60 cells,a concentration-dependent manner,and have little effectance on PBMC cells.EVn-50 decreased the number of the colony formation of HL-60 cells,a concentration-dependent manner.EVn-50 efficiently induced the apoptosis of HL-60 cells.Conclusion EVn-50 possess the effects of the growth inhibition and apoptosis induction in HL-60 cells.