脾气虚证淋巴细胞电泳测定的初步研究

本文采用方形毛细管式电泳方法对33例脾气虚证患者进行了淋巴细胞电泳观察,并与肝郁脾虚、肝胃不和型患者进行了比较。结果:脾气虚组的淋巴细胞电泳率(0.777±0.094)低于肝郁脾虚组(0.819±0.115)、肝胃不和组(0.850±0.130)和健康对照组(0.915±0.082),P<0.05～0.01;慢泳细胞电泳率也显著低于其他3组,P<0.05～0.01;快泳细胞百分数(42.1±19.3)较健康组(73.0±15.3)低,P<0.01;慢泳细胞百分数则升高(57.9±19.3),P<0.05。说明脾气虚证存在着淋巴细胞电泳能力降低,淋巴细胞快、慢泳细胞分布失常的特点。淋巴细胞电泳能力的低下,可能是脾气虚证患者免疫功能低下的机理之一。

Study on Lymphocytic Electrophoresis in Spleen-Qi Deficiency Patients

The lymphocytic electrophoresis in 33 Spleen-Qi deficiency(SQD)patients has been compared with both the patients of stagnation of Liver-Qi with deficiency of Spleen(SLQDS)and the hyper- active Liver-Qi affecting stomach(HLQAS)by means of square-capillary-type electrophoresis applying 0.145M solution of sodium chloride as the electrophoretic medium.The result showed that the electrophoretic rate of the lymphocytes of the SQD group(0.777±0.094)was lower than that of the SLQDS group(0.819±0.115),and significantly lower than that of the HLQAS group(0.850±0.130) and the normal(0.975±0.082),P<0.05,P<0.01;and the electrophoretic rate of the slow electrophor- etic lymphocytes(0.632±0.045)was also significantly lower than that of the SLQDS(0.671±0.046), HLQAS(0.669±0.045)and the normal(0.714±0.03),P<0.05,P<0.01;the percentage of the fast electrophoretic cells(42.1±19.3)was lower than normal(P<0.01),but that of the slow electrophor- etic cells(57.9±19.3)was higher(P<0.05).It revealed that the fast peak was the main peak in the normal group,the slow peak the secondary one,but on the contrary,in SQD group,the slow peak was the main and the fast peak the secondary one.The results showed that the human lymphocyte was a kind of heterogeneous cell group with different electrophoretic abilities,but in the SQD patients the heterogeneity has been changed.All these indicated that there were the characteristics of lower lymphocytic electrophoretic ability and distributing disorder of the fast and slow electro- phoretic lymphocytes in the SQD patients.The lower lymphocytic electrophoretic ability may be one of the mechanisms of the reduced immunological function in the SQD patients.