异甘草素对创伤性脑损伤大鼠损伤灶修复作用的实验研究

目的观察异甘草素对创伤『生脑损伤大鼠病理变化和血清白介素-1α（IL-1α）、IL-1β、IL-6、IL-10和肿瘤坏死因子-α（TNF-α）的影响，探讨其在创伤性脑损伤（q33I）后促进脑损伤灶康复的作用机制。方法运用改良Feeney法建立大鼠脑损伤模型，将动物随机分为假手术组、脑损伤组、异甘草素治疗组，共治疗5d，经平衡木行走试验观察神经行为学变化后处死，采用蛋白芯片法检测血清中的细胞因子，HE染色光镜下观察损伤灶的变化。结果与脑外伤组相比异甘草素治疗组IL-1α、IL-6、IL-10的血清含量明显增高，IL-1β和TNFα的含量显著降低（P〈0．05），损伤灶病理学变化明显改善。结论异甘草素可以促进创伤性脑损伤大鼠脑损伤灶康复，其机制可能与调节细胞因子有关。

Repairing effects of isoliquiritigenin on traumatic brain injury in rats

Objective To observe the effect of isoliquiritigenin on pathological changes and serum IL-1α, IL-1β, IL-6, IL-10 and TNFα after traumatic brain injury in rats and to investigate its possible mechanism for brain rehabilitation. Methods Rats were randomly divided into 3 groups： sham-operated group, traumatic brain injury （TBI） group and isoliquiritigenin-treated group. TBI model was established with the modified Feeney＇ s method. After 5 days of treatment, the TBI rats were sacrificed to measure the serum eytokines by protein chip and to observe the pathology of the brain tissues under optical microscope through HE staining. Results Compared with the TBI group, IL-1β and TNFα were decreased, IL-1 α, IL-6 and IL-10 were remarkably increased, the pathology of the brain tissues was notably alleviated. Conclusion Isoliquiritigenin can promote brain tissue rehabilitation of traumatic brain injury in rats. The underlying mechanism of this effect may be associated with the cytokines which can regulate brain tissue.