| 姜黄素对紫外线致NIH3T3细胞损伤的保护作用 |
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| 引用本文： | 张秀珍,陈中华,巨荣菊,刘东武. 姜黄素对紫外线致NIH3T3细胞损伤的保护作用[J]. 环境与职业医学, 2009, 26(3): 267-270 |
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| 作者姓名： | 张秀珍 陈中华 巨荣菊 刘东武 |
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| 作者单位： | 山东理工大学生命科学学院;山东理工大学分析测试中心; |
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| 基金项目： | 山东理工大学自然科学基金(编号:4040-306018);;山东理工大学博士启动基金项目(编号:4041-406022) |
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| 摘 要： | [目的]研究姜黄素对紫外线致NIH3T3细胞损伤的保护作用。[方法]采用四甲基偶氮唑蓝比色法（MTT试验）和激光扫描共聚焦显微镜技术研究不同浓度（1、5、10μmol／L）的姜黄素对紫外线致细胞损伤的保护作用。[结果]与紫外线照射组（UVR组）相比较，姜黄素预处理（姜黄素＋UVR）组可以提高紫外线辐照细胞的存活率（P〈0．05），减少细胞内活性氧的堆积（P〈0．05），减少细胞线粒体膜电位的下降（P〈0．05）。[结论]姜黄素对紫外线致NIH3T3细胞损伤有保护作用，其作用机制可能与清除细胞内的活性氧，维持细胞的线粒体膜电位有关。
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| 关 键 词： | 姜黄素 NIH3T3细胞 紫外线辐射 活性氧 线粒体 |
Protection Against Ultraviolet Radiation-induced Cytotoxicity by Cureumin in NIH3T3 Cells |
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| ZHANG Xiu-zhena,CHEN Zhong-huaa,JU Rong-jua,LIU Dong-wub. Protection Against Ultraviolet Radiation-induced Cytotoxicity by Cureumin in NIH3T3 Cells[J]. Journal of Environmental & Occupational Medicine, 2009, 26(3): 267-270 |
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| Authors: | ZHANG Xiu-zhena CHEN Zhong-huaa JU Rong-jua LIU Dong-wub |
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| Affiliation: | a.School of Life Sciences;b.Analysis and Testing Center;Shandong University of Technology;Zibo;Shandong 255049;China |
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| Abstract: | [Objective] To study the antioxidative effects of curcumin against damages caused by ultraviolet radiation(UVR) exposure in NIH3T3 cells.[Methods] Cell damages caused by UVR and the potential protective effects of curcumin against UVR damage to cells were monitored by detecting the cell viability using the methyl thiazolyl tetrazolium(MTT)assay,and by measuring the mitochondrial membrane potential and the cellular level of reactive oxygen species(ROS) using the laser scanning confocal microscopy.[Results] C... |
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| Keywords: | curcumin NIH3T3 cell UVR ROS mitochondria |
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