| "两袖套法"大鼠原位肝移植模型的技术改进 |
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| 引用本文： | 马跃峰,李相成,游伟. "两袖套法"大鼠原位肝移植模型的技术改进[J]. 中国现代医学杂志, 2006, 16(2): 229-232 |
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| 作者姓名： | 马跃峰 李相成 游伟 |
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| 作者单位： | 南京医科大学附属第一医院,肝脏外科,江苏,南京,210029 |
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| 摘 要： | 目的探讨建立稳定的大鼠肝脏移植模型。方法采用改进的“二套管法”对190只大鼠进行原位肝脏移植手术。结果切取供肝的时间平均为32min，修肝时间为13．5min，无肝期为17min，手术成功率达到91.7％。结论改进后的肝脏移植模型操作相对简单易学，可重复性好，模型稳定，可作为肝脏移植实验研究的有效手段。
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| 关 键 词： | 肝移植 大鼠 模型 |
| 文章编号： | 1005-8982（2006）02-0229-04 |
| 收稿时间： | 2005-04-02 |
| 修稿时间： | 2005-04-02 |
Modification of two-cuff technique orthotopic liver transplantation model |
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| MA Yue-feng,LI Xiang-cheng,YOU Wei. Modification of two-cuff technique orthotopic liver transplantation model[J]. China Journal of Modern Medicine, 2006, 16(2): 229-232 |
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| Authors: | MA Yue-feng LI Xiang-cheng YOU Wei |
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| Affiliation: | Department of Hepatic Sugery, the First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R.China |
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| Abstract: | [Objective] To modify orthotropic liver transplantation method with two-cuff technique. [Methods] orthotropic liver transplantation was conducted in 190 rats by modified cuff technique. [Results] The average time of acquiring donor liver was 32 min, repairing liver was 13.5 min, an hepatic phase was 17 min, survival rate was 91.6%. [Conclusion] The modified two-cuff technique is easily available and highly reproducible, which is practical and stable operative method of orthotopic liver transplantation in the rat. |
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| Keywords: | liver transplantation rat model |
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