Characterization of rec15, an early meiotic recombination gene in Schizosaccharomyces pombe |
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Authors: | Eveline Doll Monika Molnar Yasushi Hiraoka Jürg Kohli |
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Institution: | (1) Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH-3012 Bern, Switzerland;(2) Department of Microbiology and Biotechnology, Faculty of Sciences, University of Debrecen, 4032 Debrecen, Hungary;(3) Kansai Advanced Research Center, National Institute of Information and Communications Technology, Nishi-ku Kobe, 651-24, Japan |
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Abstract: | In S. pombe strains mutant for rec15 aberrant ascus morphology, reduced spore viability and severe reduction of meiotic recombination was detected. Genetic and
cytological analysis identified frequent interruption of meiosis after the first division, and nondisjunction I, as the main
segregation errors in the mutant. Chromosome segregation at meiosis I was not random in rec15, suggesting the presence of a backup system for correct segregation of achiasmate chromosomes. The analysis of meiotic progression
in time-course experiments revealed that the major meiotic events, such as the onset of premeiotic DNA synthesis, of horse-tail
nuclear movement, and of the first meiotic division occurred earlier in rec15 than in wild-type. The early onset of meiotic events is a novel observation for an early recombination mutant and implies
a function of rec15 protein already at or before DNA synthesis. |
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Keywords: | Fission yeast Meiosis rec15 recombination |
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