对羟基苯甲酸钠的安全药理学研究

目的 观察对羟基苯甲酸钠对实验动物中枢神经系统和心血管系统的影响。方法 昆明小鼠单次灌胃给予20，50，100 mg·kg^-1对羟基苯甲酸钠溶液，通过小鼠甩尾试验、自主活动试验、爬杆试验、协调睡眠试验和Morris水迷宫试验，考察对羟基苯甲酸钠对中枢神经系统的影响。SD大鼠单次灌胃给予14，35，70 mg·kg^-1对羟基苯甲酸钠溶液，Beagle犬单次灌胃给予4.2，10.5，21 mg·kg^-1对羟基苯甲酸钠溶液，通过检测Beagle犬血压和体温以及SD大鼠的心电，考察对羟基苯甲酸钠对心血管系统和体温的影响。结果 对羟基苯甲酸钠对小鼠感觉-运动反射、自主活动行为、协调功能、戊巴比妥钠阈下睡眠剂量小鼠入睡率和学习记忆能力无明显影响；对Beagle犬血压、体温以及SD大鼠心电均无明显影响。结论 本实验条件下，单次灌胃给予对羟基苯甲酸钠溶液对实验动物中枢神经系统和心血管系统无明显影响。

Safety Pharmacology Study of Sodium p-Hydroxybenzoate

OBJECTIVE To observe the effects of sodium p-hydroxybenzoate on the central nervous system and cardiovascular system of experimental animals. METHODS Kunming mice were given a single dose of sodium p-hydroxybenzoate of 20, 50 and 100 mg·kg^-1 by oral gavage, the effects of sodium p-hydroxybenzoate on the central nervous system were observed by mice tail-flick experiment, mice autonomic activity experiment, pole-climbing experiment, coordinating hypnosis test and Morris water maze experiment. SD rats were given a single dose of sodium p-hydroxybenzoate of 14, 35 and 70 mg·kg^-1 and Beagle dogs were given a single dose of sodium p-hydroxybenzoate of 4.2, 10.5 and 21 mg·kg^-1 by oral gavage, the effects of sodium p-hydroxybenzoate on cardiovascular system and body temperature were observed by measuring blood pressure and body temperature in Beagle dogs, and measuring electrocardiogram in SD rats. RESULTS There was no significant influence of sodium p-hydroxybenzoate on sensory-motor reflex, autonomic activity, coordinated movements, sleep rate of mice with the sub-threshold sleep dose of pentobarbital sodium and learning-memory ability. Similarly, there were no significant effects on electrocardiogram of SD rats and there were no significant effects on blood pressure and body temperature of Beagle dogs. CONCLUSION Single oral gavage of sodium p-hydroxybenzoate has no significant effects on the cardiovascular system and the central nervous system of experimental animal under the condition.