大鼠后牙牙槽骨吸收模型的建立及破骨细胞的鉴定

目的建立大鼠后牙牙槽骨吸收模型并对破骨细胞(OCs)进行鉴定。方法用3-0丝线结扎大鼠上颌第二磨牙牙颈部并喂以高糖软食,行H-E切片常规组织学观察结扎后牙周组织的变化;应用抗CTR抗体通过免疫组化的方法鉴定OCs。结果大鼠后牙结扎后第3天,结扎部位上皮糜烂,结缔组织见大量炎症细胞浸润,牙槽骨表面出现蚕食状吸收陷窝,第7天以后,结扎部位牙槽骨高度降低。在牙槽骨表面形成的骨吸收陷窝内及附近组织,可见CTR阳性的多核和单核细胞。结论丝线结扎和高糖软食的局部刺激成功地诱导了大鼠牙槽骨破骨细胞性骨吸收。应用抗CTR抗体通过免疫组化的方法鉴定OCs可用于探讨牙槽骨吸收机制的研究。

Alveolar bone resorption in rats with silk-ligature and 10% sucrose-softed diet and the identification of OCs by calcitonin receptor antibodies

Objective To build a mode of Alveolar bone resorption in rats and identify OCs by calcitonin receptor antibodies. Methods Black 3-0 gauge silk ligatures were placed around the cervix of the second maxillary molars of Sprage-Dawley rats weighing 280-300g, which were fed with 10% sucrose-softed diet. Osteoclasts and its precursor cells at periodontal tissues was identified with calcitonin receptor (CTR) immunohistochemistry. Results H-E staining of sections reveals inflammatory cell invasion and capillary dilatation in the connective tissue of the area from immediately beneath the epithelium onto which the ligature was placed, to near the alveolar bone crest on Day 3. Undermining bone resorption occurred to a large extent on the surface of alveolar bone where numerous resorption lacunae containing multinucleated cells were observed on Day 3 and Day 7. The heights of alveolar bone decreased significantly on Day 14. The multinucleated cells located in and around the alveolar bone resorption lacunae were CTR-positive. In addition, there were some mononuclear CTR-positive cells nearby, which might be osteoclast precursors. Conclusions Ligature treatment would cause alveolar bone resorption in rats fed with 10% sucrose-softed diet. The expression of CTR of osteoclasts and its precursors in periodontal tissue confirmed the involvement of osteoclasts in alveolar bone resorption.