2001年徐州地区暴发性病毒性脑膜炎病原的研究

目的 确定引起2001年江苏省徐州地区无菌性脑膜炎流行的病原体。方法 组织培养法从患者脑脊液分离病毒，标准血清中和试验鉴定分离毒株；中和试验检测双份血清中和抗体效价；逆转录聚合酶链反应（RT-PCR）检测肠道病毒特异性基因片段。结果 22份脑脊液中分离出4株柯萨奇B5型、2株柯萨奇B3型、1株艾可7型肠道病毒，分离阳性率31．8％。RT-PCR检测脑脊液21份，肠道病毒阳性11份。阳性率52．4％。19例双份血清中11例中和效价呈4倍以上增长或转阳，阳性率57．9％。结论 此次江苏省徐州地区无菌性脑膜炎流行的病原体是以柯萨奇B5为主要血清型的肠道病毒。

Etiology of aseptic meningitis prevalent in Xuzhou

Objective To find the pathogenic agents of aseptic meningitis prevalent in Xuzhou of Jiangsu province in 2001. Methods The enterovirus (EV) was cultured from CSF of the patients and identified with anti-serum by neutralization test. Neutralization titer of antibody in paired sera from meningitis children was determined. EV RNA was detected by RT-PCR. Results Four strains of Coxsackievirus B5,2 strains of Coxsackievirus B3 and 1 strain of Echovirus 7 were isolated from 22 CSF specimens. The isolation rate of virius was 31.8% (7/22),21 CSF were tested by RT-PCR,the positive rate of EV RNA was 52.4% (11/21); 57.9% (11/19) of patients'paired-sera had over 4 folds antibody rise or became seroconverted. Conclusion Enterovirus was the pathogenic agent of aseptic meningitis prevalent in Xuzhou of Jiangsu province,the main serotype of the virus was Coxsackievirus B5.