pemt2-Cdna转染抑制大鼠肝癌CBRH-7919细胞周期相关蛋白的表达

目的探讨磷脂酰乙醇胺-N-甲基转移酶2（PEMT2）的转染抑制肝癌细胞增殖的分子机制。方法将带有完整pemt2-cDNA质粒转染人大鼠肝癌CBRH-7919细胞，筛选出稳定传代并高表达PEMT2的细胞株，用Westem blot比较了细胞周期调控因子细胞周期蛋白D1／细胞周期蛋白依赖性激酶（CDK）4、细胞周期蛋白E／CDK2、phospho—Rb、caspase3、c-jun以及小窝蛋白caveolin的表达变化。结果在PEMT2高表达细胞中CDK2、CDK4、phospho—Rb和cjtin表达下调，caspase3表达上调。结论PEMT2的高表达降低cDK2和cDK4的表达水平，同时下调原癌基因phospho—Rb和c-jun的表达，抑制肝癌细胞由G1期进入S期，从而抑制肝癌细胞增殖并诱发肝癌细胞的凋亡。

Transfection of pemt-2-cDNA inhibits the expression of cell cycle related proteins in rat CBRH-7919 hepatoma cells

Objective To unravel the molecular mechanism of proliferation inhibiton reduced by transtection of pemt2- eDNA into rat CBRH-7919 hepatoma cells. Methods We started with the highly expressed PEMT2 clone. Cell culture and Western blotting techniques were used to examine the expression of cyclinD1/CDK4, cyclinFJCDK2, phospho-Rb, caspasc-3, c-jun and caveolins. Results Our results showed that CDK4, CDK2, phospho-Rb and c-jun were down regulated in the pcmt2 highly expressed cell clone. The high expression clone of pcmt2-transfected cells also showed over expression of caspase-3. Conclusion The reductions of proliferation and apoptosis of pcmt2 transfected cells could be related to the G 1 phase arrest induced by down-regulation of the cell cycle-associated proteins.