MSOME及IMSI技术在辅助生殖领域中的应用进展

精子形态与功能密切相关，精子形态异常是导致男性不育的重要原因之一。自1992年卵母细胞胞浆内单精子注射（ICSI）技术首次获得妊娠以来，许多男性因素不育夫妇通过该技术获得较为满意的妊娠结局。但在×400放大倍数下常规ICSI挑选的看似正常的精子仍可能存在超微结构的异常，这可能是导致ICSI周期失败的原因之一。活精子细胞器形态学检测（MSOME）技术是Bartoov在2001年创立的一项新技术，能在高放大倍数下更深入地观察精子细微结构，有助于挑选出形态正常的精子。精子形态选择性胞浆内单精子注射（IMSI）技术是在MSOME基础上结合传统ICSI技术发展而来，为卵母细胞ICSI提供了一个新方法。通过对MSOME和IMSI的文献进行综述，探讨其临床应用价值。

Application of MSOME and IMSI in Assisted Reproduction

Sperm morphology is closely related to male fertility. Abnormal sperm morphology is one of main causes of male infertility. Since the intracytoplasmic sperm injection（ＩＣＳＩ） was successfully developed in 1992, it has helped so many infertile couples with abnormal sperm parameters to obtain their children. Under a magnification of ×400, the only major defects in sperm morphology could be found. Those sperms with many abnormities in subcellular organelles might not be identified, which could affect the success rate of ICSI. The motile sperm organelle morphology examination（ＭＳＯＭＥ） established by Bartoov in 2001 allowed to assess the subcellular organelles of sperm under high magnification, which is helpful to pick out sperm with normal morphology. The intracytoplasmic morphologically selected sperm injection（ＩＭＳＩ）, combined MSOME with ICSI，provided a new technique for the clinical ICSI practice.