高强度聚焦超声非侵入性损伤猪膀胱壁的实验研究

目的探讨高强度聚焦超声非侵入性治疗膀胱肿瘤的可行性。方法用高强度聚焦超声对16只小型香猪膀胱各壁进行了定点辐照，观察辐照前后靶区的B超、彩超图像变化。分别于辐照后1h、4周、8周处死动物，观察靶区及膀胱周围组织肉眼、光镜及电镜变化和尿液变化。结果高强度聚焦超声能透过皮肤特异性破坏膀胱壁靶组织，导致黏膜、黏膜下层及浅肌层组织的不可逆性损伤，B超、彩超可监测到靶区的变化，4周后损伤点逐渐修复，8周后已完全被纤维结缔组织取代。结论高强度聚焦超声非侵入性治疗膀胱肿瘤是可行的。

Non-invasive Ablation of Pig Bladder Tissue Using High Intensity Focused Ultrasound:Experimental Study

Objective To investigate the feasibility of treating superficial bladder tumor with high intensity focused ultrasound(HIFU).Methods The bladder walls of 16 miniswine were irradiated with HIFU at intensity of 5832 W/cm~2 20 seconds for each target point.The irradiation effects on bladders were monitored by diagnostic ultrasound.Animals were killed within 1 hour,4 and 8 weeks after irradiation.Results HIFU penetrated the skin and precisely damaged bladder walls.Bright echoes were observed on diagnostic ultrasound in the acute lesions,but 5 minutes later,these echoes disappeared and 10 minutes later,the irradiated bladder walls became thickened.Epithelial denudation and irreversible submucosal and superficial muscular lesions were very clear under macroscopy and microscopy.Eight weeks later,the urothelium,submucosa and superficial muscles healed and replaced by fibrous connective tissues in all animals.Conclusions HIFU is a safe and effective non-invasive treatment option for superficial bladder tumors.