| 肺血栓栓塞症患者自然杀伤细胞相关基因mRNA差异性表达的意义 |
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| 引用本文： | 张晓宇,王乐民,龚朱,梁爱斌,王嫱,宋浩明.肺血栓栓塞症患者自然杀伤细胞相关基因mRNA差异性表达的意义[J].上海医学,2009,32(7). |
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| 作者姓名： | 张晓宇 王乐民 龚朱 梁爱斌 王嫱 宋浩明 |
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| 作者单位： | 1. 同济大学附属同济医院心内科,上海,200065
2. 同济大学附属同济医院血液科,上海,200065 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的 探讨自然杀伤细胞(NK细胞)在肺血栓栓塞症(PTE)过程中的作用.方法 随机入选2007年3-12月同济大学附属同济医院住院的20例PTE患者为PTE组,20例同期入院的年龄、性别与之匹配的非PTE患者为对照组.抽取外周静脉血,分离提取单个核细胞总RNA,应用基因表达谱芯片比较两组NK细胞表面受体mRNA表达的差异.结果 共筛选出NK细胞识别受体相关基因mRNA片段18条,PTE组杀伤细胞凝集素样受体(KLR)、杀伤细胞免疫球蛋白样受体(KIR)及自然细胞毒效应受体(NCR)mRNA的表达均低于对照组,其中7/9的KLR tuRNA及NCR1 tuRNA的表达显著下调(P值分别<0.05、0.01).结论 PTE过程中NK细胞的自然杀伤功能受抑制,其介导的固有免疫功能缺陷及免疫调节异常可能是获得性PTE的重要机制之一,为阐明PTE的发病机制提供了新的思路.
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| 关 键 词： | 静脉血栓栓塞症 肺血栓栓塞症 基因芯片 自然杀伤细胞 |
Differential expression of natural killer cell-associated genes in pathogenesis of pulmonary thromboembolism |
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| ZHANG Xiaoyu,WANG Lemin,GONG Zhu,LIANG Aibin,WANG Qiang,SONG Haoming.Differential expression of natural killer cell-associated genes in pathogenesis of pulmonary thromboembolism[J].Shanghai Medical Journal,2009,32(7). |
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| Authors: | ZHANG Xiaoyu WANG Lemin GONG Zhu LIANG Aibin WANG Qiang SONG Haoming |
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| Institution: | ZHANG Xiaoyu,WANG Lemin,GONG Zhu,LIANG Aibin,WANG Qiang,SONG Haoming.Department of Cardiology,Tongji Hospital,Tongji University,Shanghai 200065,China |
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| Abstract: | Objective To assess the role of natural killer cells in the pathogenesis of pulmonary thromboembolism(PTE).Methods Twenty patients with PTE were enrolled in our study and another 20 age-and sex-matched non-PTE patients served as controls.Total RNA was extracted from peripheral mononuclear cells.mRNA microarray was used to assess the differential expression of cell surface receptor mRNAs.Results A total of 18 mRNA fragments associated with surface receptors of NK cells were obtained.The mRNA expression of ki... |
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| Keywords: | Venous thromboembolism Pulmonary thromboembolism Gene chip Natural killer cell |
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