杜仲诱导大鼠bMSCs分化早期成骨/成脂相关基因表达变化

目的：观察杜仲醇提取物诱导骨髓间充质干细胞（bMSCs）分化早期成骨/成脂相关基因表达变化。方法：培养大鼠bMSCs,用杜仲醇提取物分别诱导bMSCs 8 h,1、3、7 d,采用荧光定量PCR（RT-qFCR）的方法检测成骨相关基因Id4、Runx2、ALP和成脂相关基因PPARγ、Adipoq、aP2的表达变化。结果：Id4诱导3 d后表达略有上调;Runx2基本无变化;ALP诱导7 d后显著升高;PPARγ和aP2诱导3 d达最高峰,然后下调;Adipoq诱导7 d显著上调。结论：杜仲对bMSCs的成骨/成脂分化具有双重调节作用,提示杜仲醇提取物中既含有促成骨分化,也含有促成脂分化的成分。

Expression of osteogenic and adipogenic differentiation related genes at early period in rat bMSCs induced by eucommia bark

Objective： To observe the expression of osteogenic and adipogenic differentiation related genes at early period in rat bone mesenchymal stem cells（bMSCs） induced by eucommia bark.Methods： bMSCs were incubated with MeOH extract of eucommia bark for 8 h,1 d,3 d,and 7 d.Realtime PCR was used to detect the expression of osteogenic related genes,Id4、Runx2 and ALP,and adipogenic related genes,PPARγ、Adipoq and aP2 compared to control groups.Results： Id4 upregulated slightly after induction 3 days later,Runx2 did not changed basically,ALP increased obviously after induced for 7 days,PPARγ and aP2 reached the highest point at 3 days and then downregulated and Adipoq increased obviously after induced for 7 days.Conclusion： Eucommia bark may regulate the expression of genes both osteogenic and adipogenic on bMSCs,and this implies that eucommia bark contains compositions which can promote osteoblastogenesis and adipogenesis.