| α干扰素对A_(549)细胞端粒酶活性和p53蛋白的表达 |
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| 引用本文： | 张婷,金戈,杨丽萍.α干扰素对A_(549)细胞端粒酶活性和p53蛋白的表达[J].武汉大学学报(医学版),2002,23(3):208-210. |
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| 作者姓名： | 张婷 金戈 杨丽萍 |
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| 作者单位： | 1. 河南中医学院生物化学教研室,郑州,450003
2. 郑州大学医学院生物化学教研室,郑州,450052 |
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| 基金项目： | 河南省卫生厅获奖课题，证书号 :2 0 0 13 5 |
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| 摘 要： | 目的 :探讨α 干扰素 (IFN α)对人肺腺癌细胞株A549细胞端粒酶活性和p5 3蛋白质表达的影响。方法 :用两种不同质量浓度IFN α(2 5 μg L ,10 0 μg L)处理人肺腺癌细胞株A549细胞 ,并设空白对照。采用TRAP 银染法检测端粒酶活性 ,用免疫荧光标记法测定p5 3蛋白的表达。结果 :IFN α作用后的A549细胞 ,端粒酶活性明显降低 ,且p5 3蛋白表达明显减少。结论 :IFN α能抑制肺腺癌细胞的端粒酶活性和p5 3蛋白的表达
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| 关 键 词： | 肺肿瘤 α干扰素 端粒酶 p53蛋白 |
| 修稿时间： | 2001年10月15 |
Influences of α-interferon on the Telomerase Activity and p53 Expression Telomerase Activity and p53 Expression |
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| Zhang Ting,Jin Ge,Yang Liping.Influences of α-interferon on the Telomerase Activity and p53 Expression Telomerase Activity and p53 Expression[J].Medical Journal of Wuhan University,2002,23(3):208-210. |
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| Authors: | Zhang Ting Jin Ge Yang Liping |
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| Institution: | Zhang Ting,Jin Ge,Yang Liping Department of Biochemistry,Henan Traditional Chinese Medical College,Zhengzhou 450003,China |
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| Abstract: | Objective: To investigate the influences of α interferon (IFN α) on the telomerase activity and p53 expression in A 549 alveolar carcinoma cells. Methods: A 549 alveolar carcinoma cells were treated with IFN α(25 μg/L,100 μg/L). p53 expression and the telomerase activity were determined with immunohistochemisty and the non radioactive method (silver staining method)in the course of differentiation in A 549 cells. Results: Both p53 expression and the telomerase activity were significantly decreased in the A 549 cell line treated with IFN α. Conclusion: IFN α could inhibit the telomerase activity and the p53 expression of A 549 cells. |
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| Keywords: | lung neoplasm interferon alpha telomerase protein p53 |
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