Molecular identification of interleukin-2 in the lymphoid tissues of the common brushtail possum, Trichosurus vulpecula |
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Authors: | Young L J Cross M L Duckworth J A Flenady S Belov K |
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Institution: | a Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia b Pest Control Technologies Team & NRC Possum Biocontrol at Landcare Research, Lincoln, New Zealand c Centre for Environmental Management, Central Queensland University, Rockhampton, Queensland, Australia |
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Abstract: | The common brushtail possum (Trichosurus vulpecula) is an Australian marsupial. Here we describe the identification of possum interleukin-2 in mitogen-stimulated lymph node cells. We used a strategy of Rapid amplification of cDNA ends using probes designed from recently-sequenced marsupial genomes to identify the IL2 gene and then confirmed that IL-2 expression in possum immune tissue occurs in a similar manner to that in their eutherian counterparts. The predictive possum IL-2 peptide showed 28% and 35% amino acid sequence homology with the mouse and human IL-2 molecules, respectively, consistent with the divergence found within this cytokine family. Despite this low sequence identity, possum IL-2 still possessed the characteristic hallmarks of mammalian IL-2, such as a predicted signal peptide and conserved family motifs. |
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Keywords: | Brushtail possum Cytokine Interleukin-2 Marsupial Trichosurus vulpecula |
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