红枣汁对小鼠血脂水平和身体机能的影响

背景红枣中含一定量的环磷酸腺苷和环磷酸鸟苷,对高血压、糖尿病、癌症、心源性休克等有一定疗效.而红枣对血脂影响的报道结果较少. 目的探讨红枣汁对小鼠身体机能和血脂水平的影响. 设计以实验动物为研究对象,完全随机分组设计. 单位一所大学的生命科学与技术学院生物工程系,一所大学医院的内分泌科. 材料实验于 2003- 03/05在西安交通大学生命科学与技术学院生物工程系完成,选择清洁级 ICR种小鼠,体质量 20～ 24 g.陕西省中医药研究院提供 陕医动(字) 08- 004].红枣汁陕北木枣,经挑选、洗涤、热处理、适度破碎,加入 10倍水、质量分数为 0.70%果胶酶,于 50 ℃下恒温浸泡 6 h,过滤,滤液浓缩至可溶性固形物含量为 16% ,杀菌备用.取 48只小鼠,雌雄各 24只,按体质量随机分 4组,各 12只,包括正常对照组,正常给枣汁组,模型对照组,模型给枣汁组. 方法正常对照组饲以基础饲料、 24 h给水;正常给枣汁组饲以基础饲料、 12 h给水、 12 h给枣汁;模型对照组饲以高脂饲料、 24 h给水;模型给枣汁组饲以高脂饲料、 12 h给水、 12 h给枣汁.测定红枣汁喂养 30 d后小鼠的体质量变化、胸腺、脾脏指数,用高脂饲料造成小鼠高脂模型,测定血脂指标. 主要观察指标小鼠食物功效、胸腺指数、脾指数,小鼠生长指标. 结果给枣汁组食物功效、胸腺指数、脾脏指数分别( 9.93± 0.473) g/100 g, 3.27± 0.35, 2.93± 0.51均显著高于对照组( 7.56± 0.382) g/100 g, 3.14± 0.54, 2.87± 0.33( t=2.55, 2.37, 2.41, P< 0.05);给枣汁组体质量( 36.73± 4.70) g显著高于对照组 (34.92± 5.12) g( t =2.32, P< 0.05),模型对照组、模型给枣汁组体质量( 39.83± 6.00) g,( 37.63± 2.73) g极显著高于对照组和给枣汁组( 34.92± 5.12) g,( 36.73± 4.70) g( t=4.13,2.48, P< 0.01),而模型给枣汁组体质量( 37.63± 2.73) g又显著低于模型对照组( 39.83± 6.00) g( t=2.26,P< 0.05);模型对照组、模型给枣汁组总胆固醇含量均极显著高于对照组和给枣汁组( P< 0.01);而模型给枣汁组总胆固醇含量显著低于模型对照组( P< 0.05) ; 模型对照组三酰甘油、低密度脂蛋白胆固醇含量、动脉硬化指数、高密度脂蛋白胆固醇含量、高密度脂蛋白胆固醇 /总胆固醇显著高于对照组( P< 0.01,P< 0.05);模型给枣汁组总胆固醇、高密度脂蛋白胆固醇含量、动脉硬化指数、高密度脂蛋白胆固醇 /总胆固醇、三酰甘油、低密度脂蛋白胆固醇含量低于模型对照组( P< 0.05, P< 0.01). 结论红枣汁可明显增加小鼠体质量,增加食物功效,增大胸腺、脾脏指数,对小鼠身体机能有显著的增强作用;红枣汁可降低高血脂症小鼠的血清总胆固醇、血清三酰甘油、血清低密度脂蛋白,对高脂饲料所致小鼠的高血脂症有显著的改善作用

Effects of juice of Fructus Zipiphi Jujubae on blood lipid level and body function in mice

BACKGROUND:Fructus Zipiphi Jujubae,with certain cyclic adenosine monophosphate(cAMP) and cyclic guanosine monophosphate(cGMP),has effects on hypertension,diabetes,cancer and cardiogenic shock.But there has been rare report on the influence of Fructus Zipiphi Jujubae on blood lipid. OBJECTIVE:To study the effects of juice of Fructus Zipiphi Jujubae on body function and blood lipid level in mice. DESIGN:A completely randomized basic study taking experimental animals as the subjects. SETTINGS:Department of bioengineering,school of life science and technology in a university;Department of endocrinology in a university hospital. PARTICIPANTS:The experiment was completed in the Department of Bioengineering,School of Life Science and Technology in Xi'an Jiaotong University from March to May 2003.ICR mice of clean grade, weighing 20 g to 40 g,were provided by the Shaanxi Academy of Traditional Chinese MedicineCertification No.08-004]. Juice of Fructus Zipiphi Jujubae:The Chinese date was picked, cleaned,heated and fractured moderately,mixed with water of 10 times and pectinase with the quantity percentage of 0.70% ,soaked at 50 ℃ for 6 hours,then the juice was filtered and condensed into 16% soluble solids,then sterilized for use later.Forty-eight mice (24 male and 24 female) were randomly divided into 4 groups according to body mass with 12 in each group:normal control group(group A), normal date juice group(group B),model control group(group C) and model date juice group(group D). INTERVENTIONS:Mice in group A were fed with basic diet,drank water for 24 hours;Mice in group B were fed with basic diet,drank water and date juice for 12 hours respectively;Mice in group C were fed with high-fat diet,drank water for 24 hours;Mice in group D were fed with high-fat diet,drank water and date juice for 12 hours respectively.Changes of body mass,indexes of thymus gland and spleen were measured after treated with date juice for 30 days.High fat mice models were established,and blood lipid indexes were determined. MAIN OUTCOME MEASURES:Food efficiency of mice,index of thymus gland,spleen index,growth index of mice. RESULTS:The food efficiency and the indexes of thymus gland and spleen in group B were(9.93± 0.473) g/100 g,3.27± 0.35 and 2.93± 0.51 respectively,which were all obviously higher than those in group A(7.56± 0.382) g/100 g,(3.14± 0.54),(2.87± 0.33)](t=2.55,2.37,2.41,P< 0.05).The body mass was obviously higher in group B (36.73 ± 4.70) g] than in group A(34.92 ± 5.12) g] (t=2.32,P< 0.05);while those were very significantly higher in group C and group D (39.83 ± 6.00), (37.63 ± 2.73) g] than in group A and group B(34.92 ± 5.12), (36.73 ± 4.70) g](t=4.13,2.48,P< 0.01),and that in the group D(37.63 ± 2.73) g] was obviously lighter than that in the group C(39.83 ± 6.00)g](t=2.26,P< 0.05).The serum levels of total cholesterol(TC) in the in group C and group D were significantly higher than those in group A and group B(P< 0.01), while that was significantly lower in group D than in group C(P< 0.05). The serum levels of triglyceride(TG),low-density lipoprotein-cholesterol(LDL-C),arteriosclerosis index(AI),high-density lipoprotein-cholesterol(HDL-C) and HDL-C/TC were significantly higher in group C than in group A(P< 0.01,P< 0.05).TC, HDL-C,AI,HDL-C/TC,TG and LDL-C in group D were lower than those in group C(P< 0.05,P< 0.01). CONCLUSION:Juice of Fructus Zipiphi Jujubae can obviously increase the body mass,promoting food efficiency and indexes of thymus gland and spleen of mice, and has the significant effect on the reinforcement of body function in mice.Juice of Fructus Zipiphi Jujubae can decrease the serum levels of TC,TG and LDL-C in mice with hyperlipidemia,and has the remarkable ameliorating effect on hyperlipidemia of mice induced by high-fat feed.