孕妇血清、乳汁及新生儿脐血中输血传播病毒感染的研究

目的 了解输血传播病毒（TTV）在孕妇血清、乳汁及新生儿脐血中的感染状况，探讨TTV的传播途径。方法 用半巢式聚合酶链反应方法对150例孕妇血清、乳汁及新生儿脐血标本，进行TTV DNA扩增，对8例PCR阳性扩增产物再进行直接测序。结果 （1）150例孕妇血清TTV DNA的阳性率为11．3％（17／150），17例孕妇血清TTV DNA阳性者中，乳汁阳性11例，其新生儿脐血均为阴性。（2）检测3例孕妇血清TTV DNA序列及乳汁TTV DNA序列，其核苷酸同源性为97．6％－99．2％；5例孕妇血清TTV DNA序列之间同源性为71．5％－92．8％，与日本原型株NSS的核苷酸及氨基酸序列同源性为71．5％－92．8％。结论 孕妇血清及乳汁中存在TTV感染，经乳汁传播可能是该病毒感染的主要方式之一。

Study on infection of transfusion transmitted virus in serum, breast milk of pregnant women and umbilical venous blood from their newborns

OBJECTIVE: To study transfusion transmitted virus (TTV) infection in pregnant women and their newborns, and the route of transmission. METHODS: Serum, breast milk from 150 pregnant women and umbilical venous blood from their newborns were collected. TTV DNA was amplified by semi-nested-polymerase chain reaction, and the positive products were sequenced directly. RESULTS: The positive rate of TTV DNA in pregnancy serum was 11.3% (17/150). Among the 17 serum positive cases, TTV DNA was detected in 11 breast milk samples, and none in all the umbilical blood. The homologous of partial nucleotide sequence of TTV DNA between the 3 serum samples and their breast milk were 97.6%-99.2%. Compared the 5 serum positive samples (A1-A5) with N22 segments of Japanese original isolate, the homologous of partial nucleotide and amino acid sequence were 71.5%-92.8%, 71.5%-92.8%, respectively. CONCLUSION: There exists TTV infection in pregnancy serum and breast milk. Transmission by breast feeding may be one of the main pathway causing TTV infection.