D-半乳糖衰老模型观察的新指标

目的观察D-半乳糖衰老模型与正常小鼠之间衰老指标的差别.方法采用D-半乳糖sc造成衰老小鼠模型,测定脑组织Ca2 含量、Ca2 -ATP酶(钙泵)、Na -ATP酶(钠泵)、NO和AngⅡ胰岛素(ISN)活性.结果D-半乳糖衰老模型小鼠与对照组比较脑组织Ca2 含量(P＜0.01)、NO水平(P＜0.05)明显增加;Ca2 -ATP酶、Na -ATP酶活性下降(P＜0.01),胰岛素、AngⅡ水平明显下降(P＜0.01),Zn2 /Cu2 比值有显著性差异.结论D-半乳糖衰老模型小鼠与正常小鼠之间在组织Ca2 含量、Ca2 -ATP酶(钙泵)、Na -ATP酶(钠泵)、NO、AngⅡ和胰岛素活性有显著性差异,Zn2 /Cu2 比值下降,可作为抗衰老药物研究的模型.

New indices in tissue of senile model

OBJECTIVE To study the in dices difference of senile mouse model induced by D-galactose and normal mouse.METHOD Senile mouse model was induced by injecting subcutaneously D-galactose. The content of Ca 2 , Ca 2 -Mg 2 -ATP ase, Na -K -ATP ase, NO, AngII and insulin(ISN) in brain were determined.RESULTS Compared with the normal, the content of Ca 2 and NO in brain were increased (P<0.05;P<0.01) and Ca 2 -Mg 2 -ATP ase, Na -K -ATP ase, AngII and ISN were decreased(P<0.01) in senile mouse model. And there existed obvious difference in the ratio of Zn 2 /Cu 2 and immunologic function.CONCLUSION There existed obvious difference in the content of Ca 2 , Ca 2 -Mg 2 -ATP ase, Na -K -ATP ase, NO, AngII and insulin(ISN) in brain and the ratio of Zn 2 /Cu 2 ,immunologic function between the senile mouse model and normal. These indices can be new indices in tissue of senile model.