不同保存介质对脐带间充质干细胞活性的影响

背景：脐带间充质干细胞的临床应用面临移植前保存的问题，而临床保存介质对移植前细胞活性的影响直接关乎移植后疗效，目前却缺乏对临床介质保存效果的系统性研究。目的：评价临床常用保存介质对脐带间充质干细胞活性的影响。方法：在4℃与室温（25℃）条件下，将脐带间充质干细胞在临床常用保存液（0．9％氯化钠注射液，5％葡萄糖注射液和1％人血白蛋白溶液）中保存2，4，6h，观察上述保存介质对脐带间充质干细胞活性、凋亡，死亡率、多向分化能力及DNA损伤的影响。结果与结论：无论在4℃还是室温条件下，细胞在临床常用保存介质中的活性均在短期内呈时间依赖性下降。此外，不同保存介质引起细胞死亡而非凋亡，且死亡率呈时间依赖性增加；经不同介质保存后，细胞出现DNA损伤，但其增殖与多向分化能力未受明显影响，提示细胞核DNA损伤为细胞活性下降的关键因素。

Effects of different storage solutions on the viability of umbilical cord mesenchymal stem cells

BACKGROUND： Umbilical cord-mesenchymal stem cells （UC-MSCs） have been widely studied in clinics. Notably, UC-MSCs have to be properly preserved before clinical use and the effects of storage solution on viability of cells play critical roles in the effectiveness of cell-transplantation. However, there are few studies concerning this phenomenon. OBJECTIVE： To evaluate the effects of various clinical common solutions on the activity of UC-MSCs. METHODS： UC-MSCs were stored at 0,9% sodium chloride injection, 5% glucose injection and 1% human serum albumin respectively at 4 ~C and room temperature （25 ~C） for 2, 4 or 6 hours. Viabilities, apoptosis/necrosis rate multi-differentiation ability of preserved UC-MSCs and DNA damage were examined. RESULTS AND CONCLUSION： The viabilities of UC-MSCs were rapidly decreased time-dependently in all of the storage solutions both at 4 ~C and room temperature. Besides, the storage solutions resulted in cellular necrosis rather than apoptosis and the necrosis rate was greatly increased time-dependently. DNA damage was observed in cells after preserved in various storage solutions, but cell proliferation and differentiation were not affected, which demonstrated that DNA damage may be a key factor for the reduction of cell viabilities.