构建糖尿病膀胱病豚鼠模型及其尿动力学评价

背景：糖尿病性膀胱病是糖尿病患者最常见的慢性并发症之一，建立糖尿病膀胱病动物模型为相关研究提供实验动物平台。 目的：建立糖尿病膀胱病豚鼠模型并进行尿动力学评价。 方法：50只英国种短毛雌性豚鼠，实验组（n=42）以单次腹腔注射链脲佐菌素法诱导糖尿病豚鼠，对照组（n=8）注射相应剂量空白枸橼酸缓冲液，分别在9周和12周时行尿动力学检查，确定膀胱功能失代偿豚鼠即糖尿病性膀胱病组和代偿豚鼠即代偿组豚鼠尿动力学特点。 结果与结论：42只豚鼠有20只成功诱导出糖尿病。糖尿病豚鼠中，9周时9只糖尿病组豚鼠，6只膀胱功能代偿，3只失代偿；12周时，另外9只糖尿病组豚鼠，1只膀胱功能代偿，8只失代偿（89%）。糖尿病性膀胱病组豚鼠残余尿量增加（0.72±0.08） mL、最大逼尿肌压下降（0.63±0.05） kPa、膀胱容量增加（2.01±0.05） mL及膀胱顺应性增加（3.47±0.41） mL/kPa，与对照组及代偿组比较，差异均存在显著性意义（P 〈0.001）。豚鼠一般可在诱导糖尿病成功后12周发生糖尿病性膀胱病，表现出膀胱残余尿量增加等相应的尿动力学改变。

Establishment of diabetic cystopathy guinea pig model and its urodynamic evaluation

BACKGROUND：Diabetic cystopathy is one of the most common chronic diabetic complications. The establishment of animal models of diabetic cystopathy wil provide experimental animal platform for relevant research. OBJECTIVE：To establish a guinea pig model of diabetic cystopathy and to evaluate its urodynamic characteristics. METHODS：Fifty short-hair Britain female guinea pigs were randomly divided into two groups, 42 as the experiment group and the other 8 as the control group. The experiment group was intraperitoneal y injected with streptozotocin to induce diabetes. The control group received injection of blank citric acid buffered solution. Diabetic guinea pigs were detected by urinary dynamics test at 9 and 12 weeks. Diabetic guinea pigs were further assigned into diabetic cystopathy subgroup and compensated subgroup. The urodynamic parameters of three groups were compared. RESULTS AND CONCLUSION：Twenty of 42 guinea pigs were successful y induced diabetes by the injection of＆nbsp;streptozotocin. At 9 weeks after the injection, bladder function compensation was present in six diabetic guinea pigs while bladder function was decompensated in another three diabetic guinea pigs. At 12 weeks, bladder function compensation was present in one diabetic guinea pig, while another eight guinea pigs were confirmed with diabetic cystopathy （88.89%）. In the diabetic cystopathy subgroup, the residual urine volume was increased （0.72±0.08） mL, maximal detrusor pressure was decreased （0.63±0.05） kPa, maximum bladder capacity was increased （2.01±0.05） mL, and bladder compliance was increased （0.34±0.04） mL/kPa. There were significant differences compared with the compensated subgroup and the control group （P〈0.001）. Diabetic cystopathy occurs at 12 weeks after diabetic models are successful y established in guinea pigs, and urodynamic changes are mainly the increase of residual urine volume.