基质结构蛋白在脑血管畸形中的表达差异

背景：有研究表明脑血管畸形中存在管壁结构基质蛋白的表达差异,但血管畸形间结构基质蛋白的差异及结构蛋白差异与畸形血管管壁异形关系少有报道.目的：观察基质结构蛋白在脑血管畸形中的差异性表达.方法：对50例脑血管畸形手术标本和34例正常颞浅动脉标本进行苏木精-伊红染色,并应用美国Santa Cruz公司生产单/多克隆抗体对4种基质结构蛋白-Ⅳ型胶原蛋白、a-血管平滑肌蛋白、层粘连蛋白和纤维连接蛋白免疫组织化学染色.结果与结论：苏木精-伊红染色显示,脑血管畸形壁结构与正常血管相比呈现明显紊乱和构成异形性;且脑血管畸形中,脑动静脉畸形与海绵状血管瘤结构存在明显差异.免疫组织化学染色显示：正常颞浅动脉和畸形血管中Ⅳ型胶原和a-血管平滑肌蛋白全部呈阳性表达且无显著性差异,但结构排列差异明显;正常颞浅动脉与畸形血管层粘连蛋白和纤维连接蛋白表达阳性率差异有显著性意义（P＜0.05）,正常血管表达更多层粘连蛋白,畸形血管表达更多纤维连接蛋白;畸形血管中脑动静脉畸形和海绵状血管瘤间层粘连蛋白和纤维连接蛋白的阳性率表达差异有显著性意义（P＜0.05）,脑动静脉畸形血管表达更多层粘连蛋白,海绵状血管瘤血管表达更多纤维连接蛋白.结果证实：与正常血管相比,脑血管畸形者管壁结构存在明显异形性,结构蛋白Ⅳ型胶原和a-血管平滑肌蛋白结构明显紊乱,且低表达层粘连蛋白而高表达纤维连接蛋白,这种差异可能是导致脑血管壁结构异常的原因.

Differential expression of matrix structural proteins in cerebrovascular malformations

BACKGROUND： Studies have shown that the wall structure matrix protein expression has some differences in brain vascular mafformations, but the difference between the structure matrix proteins of vascular mafformations and the correlation between structural protein difference and vascular mafformations are rarely reported. OBJECTIVE： To evaluate the differentialexpression of matrix structural proteins in vascular mafformations of the brain. METHODS： Fifty surgical specimens of cerebral vascular mafformation and 34 normal specimens of superficial temporal artery were subject to hematoxylin-eosin staining. Single/polyclonal antibodies, produced by Santa Cruz USA against four structural proteins matdx （IV collagen, a-smooth muscle protein, laminin, fibronectin） were applied for immunohistochemical staining. RESULTS AND CONCLUSION： Hematoxylin-eosin staining showed that, cerebral vascular malformations showed significant disorder and constitute heteromorphism compared with normal vascular wall structure; in vascular malformations, there were obvious differences in cerebral arteriovenous malformations and cavernous hemangioma structure. Immunohistochemical staining showed that, in the normal temporal artery and vascularmalformations, IV collagen and smooth muscle actin-a were both positively expressed, and the positive rate had no difference, but the differences were significant in the structural arrangement. Laminin and fibrenectin expression rate showed statistically significant differences between normal temporal artery and vascular malformations （P 〈 0.05）, normal vascular wall expressed more laminin, while vascular malformations expressed more fibronectin. Laminin and fibronectin expression rate also had significant differences between cerebral arteriovenous malformation and cavernous hemangioma （P 〈 0.05）, cerebral arteriovenous malformation expressed more laminin, while cavernous hemangioma expressed more fibronectin. The results confirmed that, compared with normal cerebral vessels, cere