脂肪干细胞三维生长骨组织工程复合体的构建

背景：组织工程支架是模仿细胞赖以生长代谢的细胞外基质而构建的支架和环境,其选择、制备以及种子细胞的选择是骨组织工程领域中的一项十分重要的课题.目的：利用几丁质凝胶/异种骨构建脂肪干细胞三维生长环境,并对其相容性进行研究.方法：从出生8d新西兰大白兔腹股沟获取脂肪组织,提取脂肪干细胞.脂肪干细胞经过体外成骨诱导分化后,种植于几丁质凝胶/异种骨,构建新型骨组织工程复合体,并将其设为细胞/几丁质凝胶/异种骨组;将脂肪干细胞直接种植于异种骨,构成脂肪干细胞/异种骨复合体作为细胞/异种骨组,单独异种骨为空白组.体外诱导2周后进行电镜扫描,观察细胞与支架的复合情况.结果与结论：镜扫描观察显示几丁质凝胶充分渗透于支架的空隙内,形成一个细胞的三维生长环境,使原本只能在材料上贴壁生长的脂肪干细胞能够在三维的环境中生长,为细胞外基质的再生提供足够的空间.几丁质凝胶/异种骨悬浮诱导后的脂肪干细胞,承载了更多的细胞,减少了细胞在载体中的流失,是一种较好的骨组织工程载体.

Three-dimensional growth of adipose-derived stem cells in bone tissue engineering complex

BACKGROUND： Tissue engineering scaffolds are designed to imitate extracallular matrix, which is essential for cellular growth and metabolism. The scaffold preparation and the selection of seed calls are the hot topics in bone tissue engineering field. OBJECTIVE： To construct three-dimensional growth environment of adipose-derived stem cells with the chitosan/xenogeneic bone, and investigate its compatibility. METHODS： Adipose tissue was obtained from New Zealand white rabbits at 8 days old, and adipose-derived stem cells were extracted. After induction of osteogenic differentiation in vitro, the cells were cultured on chitosan/xenogenic bone, to construct bone tissue engineering complex, serving as the experimental group. The adipose-derived stem cells were directly cultured in xenogenic bone, to construct calls/xenogeneic bone complex, serving as a control group. The simple bone xenograft served as a blank group. Two weeks after in vitro induced differentiation, the complex or xenograft in three groups were observed under scanning electron microscope to observe the cells and scaffold compound the situation, RESULTS AND CONCLUSION： The Chitosan fully penetrated into the scaffolds, and formed a three-dimensional environment for call growth, so that adipose-derived stem cells can grow in the three-dimensional environment,which provide sufficient space for the regeneration of the extracellular matrix. After induction of the chitosan/xenogenic bone, adipose-derived stem cells can carry more cells, and the loss of cells in the vector is reduced, so it is a good carrier of bone tissue engineering.