尼古丁对不同表面处理种植体骨结合的影响简

背景：研究证实尼古丁会影响成骨细胞、破骨细胞、成纤维细胞和红细胞的活性。 目的：检测尼古丁对表面喷砂或酸蚀处理种植体植入后骨结合及骨保护素、骨形成蛋白2表达的影响。 方法：将24只SD大鼠随机均分为实验组和对照组，实验组大鼠每天2次背部皮下注射2 mg/kg尼古丁，对照组对应皮下注射等量生理盐水。2周后在两组大鼠胫骨近干骺端分别植入表面喷砂或酸蚀处理的钛种植体，实验组继续皮下注射尼古丁，对照组注射生理盐水。种植后第2，4周对种植体及其周围骨组织行CT、X射线、荧光定量PCR及苏木精-伊红染色观察。 结果与结论：与对照组相比，实验组骨结合程度及骨保护素和骨形成蛋白2表达明显下降（P 〈0.05）。在尼古丁作用下，表面喷砂处理种植体组骨保护素和骨形成蛋白2表达、表面酸蚀处理种植体组骨保护素表达均随时间变化明显下调（P 〈0.05），并且表面酸蚀处理种植体组植入2周时骨形成蛋白2表达高于表面喷砂处理种植体组（P 〈0.05）；X射线与CT结果提示，尼古丁干预对表面酸蚀处理种植体周围新生骨形成量和新生骨矿化程度的影响明显小于表面喷砂处理种植体。苏木精-伊红染色显示，两种种植体周围成骨细胞的数量与活性随时间变化均降低，但表面酸蚀处理种植体组效果好于表面喷砂处理种植体组。

Nicotine effects on the osseointegration of implants with different treatments

BACKGROUND：Studies have confirmed that nicotine affects the activity of osteoblasts, osteoclasts, fibroblasts and erythrocytes. OBJECTIVE：To study the nicotine effects on osseointegration and the expression of osteoprotegerin and bone morphogenetic protein 2 after implantation of dental implants with surface treatment by sandblasting or acid etching. METHODS：Twenty-four Sprague-Dawley rats were randomly assigned to two groups and received daily injections for 2 weeks as folows： Nicotine 2 mg/kg twice for experimental group, saline solution for control group. Then the titanium implants with surface sandblasting or acid etching were implanted into the tibiae folowed by continuous nicotine or normal saline injection. At weeks 2 and 4 after implantation, the implants and surrounding bone tissue were prepared for CT, X-ray and hematoxylin-eosin staining examinations to evaluate bone healing and expression levels of bone-related genes were measured by quantitative RT-PCR. RESULTS AND CONCLUSION： Compared with the control groups, the degree of osseointegration and the expression of osteoprotegerin and bone morphogenetic protein 2 in the experimental groups were decreased significantly （P 〈 0.05）, except that the expression of bone morphogenetic protein 2 in the experimental group with acid etching was not significantly reduced. In addition, the expression of bone morphogenetic protein 2 in the experimental group with acid etching was higher than that in the experimental group with sandblasting at 2 weeks after implantation （P 〈 0.05）. The X-ray and CT show that the quantities of new generation bone and the degree of bone mineralization of the sandblasting group were significant lower than those of the acid etching group under the intervention of nicotine. Hematoxylin-eosin staining showed that the activity and quantity of osteoblasts around the implants down-regulated significantly, but acid etching-treated implants showed better outcomes than sandblasting-treated implants.