胆囊收缩素促坐骨神经再生的可能机制

背景：前期实验发现，八肽胆囊收缩素能促进大鼠坐骨神经损伤后的再生，但具体机制仍不清楚。目的：筛选有效指标，尝试从神经生长因子及神经再生微环境的角度分析八肽胆囊收缩素促进大鼠坐骨神经再生的机制。方法：选择健康SD大鼠，制备坐骨神经单侧离断伤模型后随机分为2组，八肽胆囊收缩素治疗组造模后连续7 d腹腔注射八肽胆囊收缩素8 nmol/kg，对照组腹腔注射等量生理盐水。检测两组大鼠局部神经生长因子蛋白表达、脊髓诱导型一氧化氮合酶水平、血清超氧化物歧化酶活性和丙二醛浓度，同时检测脊髓凋亡细胞数。结果与结论：八肽胆囊收缩素治疗组大鼠局部神经生长因子蛋白表达高于对照组（P＜0.01），脊髓诱导型一氧化氮合酶和凋亡细胞数低于对照组（P＜0.01），血清超氧化物歧化酶活性高于对照组且丙二醛浓度低于对照组（P＜0.01，0.05）。说明胆囊收缩素促进坐骨神经再生的可能机制包括保护神经元、抗细胞凋亡、抑制炎性反应、抗NO及氧化反应、抗丙二醛减轻自由基损伤等方面外，还可刺激神经生长因子的表达和释放。

Possible mechanisms of cholecystokinin promoting sciatic nerve regeneration

BACKGROUND：Previous studies have found that cholecystokinin octapeptide （CCK-8） can promote the regeneration after sciatic nerve injury in rats, but the exact mechanism remains unclear.OBJECTIVE：To screen effective indicators and analyze the mechanism of CCK-8 promoting sciatic nerve regeneration from the perspective of nerve growth factor and nerve regeneration microenvironment.METHODS：Healthy Sprague-Dawley rats, for the preparation of unilateral sciatic nerve transection injury model, were randomly divided into two groups. In the CCK-8 group, the animal model received intraperitoneal injection of CCK-8 （8 nmol/kg） for consecutive 7 days, while the control group was injected with equal volume of normal saline. The nerve growth factor expression, inducible nitric oxide synthase in the spinal cord, serum superoxide dismutase activity and malondialdehyde concentration, as wel as apoptotic cel s in spinal cord were al detected.RESULTS AND CONCLUSION：In the CCK-8 group, nerve growth factor expression was higher than that in the control group （P〈0.01）, while inducible nitric oxide synthase and the number of apoptotic cel s were lower （P〈0.01）, serum superoxide dismutase activity was higher but malondialdehyde concentrations was lower （P〈0.01, 0.05）. The mechanisms of CCK-8 promoting sciatic nerve regeneration include protecting neurons, anti-apoptosis,＆amp;nbsp;inhibiting inflammatory response, anti-NO and anti-oxidation, reducing malondialdehyde, and al eviating free radical damage, as wel as stimulating nerve growth factor expression and release.