C57小鼠脂肪及骨髓间充质干细胞生物学特性的比较

背景：研究发现，脂肪源干细胞具有和骨髓间充质干细胞一样的贴壁和形成成纤维样克隆特性，并具有向骨、脂肪、软骨等多系分化的能力。 目的：比较C57小鼠脂肪源间充质干细胞和骨髓间充质干细胞的生物学特点。 方法：在无菌的条件下分别从C57小鼠的脂肪和骨髓中获取脂肪间充质干细胞和骨髓间充质干细胞。体外分离、培养并将脂肪间充质干细胞和骨髓间充质干细胞传至第3代，进行细胞形态、表面标记、生长动力学分化潜能测定和Notch信号相关基因的检测。 结果与结论：脂肪间充质干细胞和骨髓间充质干细胞形态学相似，第3代的脂肪间充质干细胞和骨髓间充质干细胞均表达CD29、CD105、Sca-1，不表达CD34、CD133，但骨髓间充质干细胞还表达CD45；生长曲线和细胞克隆分析显示脂肪间充质干细胞的增殖速度明显比骨髓间充质干细胞快；脂肪间充质干细胞和骨髓间充质干细胞均可向成骨、成脂、成软骨诱导分化，脂肪间充质干细胞更易向成骨诱导；Notch 相关基因检测显示脂肪源干细胞的 Jagged-1表达水平明显比骨髓间充质干细胞低，而 Hes-1的表达水平脂肪源干细胞明显高于骨髓间充质干细胞的表达水平。提示脂肪间充质干细胞比骨髓间充质干细胞扩增能力更强，更易向成骨分化,可能与Hes-1表达水平有关。

Biological characteristics of bone marrowversus adipose-derived mesenchymal stem cells from C57 mice

BACKGROUND：Adipose-derived stem cels have similar characteristics of bone marrow mesenchymal stem cels, including adhesion and fibroblast-like clone formation. In addition, adipose-derived stem cels can differentiate into bone, adipose, cartilage.
OBJECTIVE：To compare the biological characteristics of C57 mouse adipose-derived and bone marrow mesenchymal stem cels.
METHODS：Mesenchymal stem cels were obtained respectively from the adipose and bone marrow of C57 mice under sterile condition. Adipose-derived and bone marrow mesenchymal stem cels were isolated and purified in vitro to determine cellmorphology, suface marker, growth kinetics, differentiation potential and Notch signal related gene.
RESULTS AND CONCLUSION：Morphology was similar between adipose-derived and bone marrow mesenchymal stem cels under an optical microscope. Adipose-derived and bone marrow mesenchymal stem cels at passage 3 expressed CD29, CD105, Sca-1, but not expressed CD34, CD133. In addition, bone marrow mesenchymal stem cels also expressed CD45. The growth kinetics and cellclone analysis indicated that the proliferation rate of adipose-derived mesenchymal stem cels was significantly faster than that of bone marrow mesenchymal stem cels. Both of adipose-derived and bone marrow mesenchymal stem cels were induced to osteoblasts, adipocytes and chondrocytes, but the adipose-derived mesenchymal stem cels were easier to the osteogenetic differentiation than bone marrow mesenchymal stem cels. Notch signal related gene detection shown that adipose-derived mesenchymal stem cels expressed lower levels of Jagged-1 mRNA, but higher levels of Hes-1 mRNA compared with bone marrow mesenchymal stem cels. These findings suggest that the proliferation rate of adipose-derived mesenchymal stem cels is significantly faster than that of bone marrow mesenchymal stem cels, and the former is apt to the osteogenetic differentiation and may be related with the expressed levels of Hes-1 mRNA.