芬戈莫德联合骨髓间充质干细胞移植脑创伤大鼠记忆功能的变化

背景：单纯骨髓间充质干细胞移植对脑损伤的修复作用并不理想，需要结合药物及生物工程材料等手段进行综合治疗。目的：观察骨髓间充质干细胞移植同时应用芬戈莫德免疫抑制剂对脑损伤大鼠记忆功能恢复的影响。方法：选取健康SD大鼠60只，采用液压颅脑损伤仪，给予253.3125-303.975 kPa峰值的液压冲击力，制成重型液压颅脑损伤模型，随机分成为脑损伤组、骨髓间充质干细胞移植组、芬戈莫德＋骨髓间充质干细胞移植组。PKH-26标记的骨髓间充质干细胞移植后21-28 d进行Morris水迷宫试验。脑损伤4周后取脑组织行PKH-26免疫荧光、苏木精-伊红染色。结果与结论：移植后4周，各组平均潜伏时间均逐渐缩短，芬戈莫德＋骨髓间充质干细胞移植组平均潜伏时间较骨髓间充质干细胞移植组缩短（P＜0.05），较脑损伤组明显缩短（P＜0.01）。芬戈莫德＋骨髓间充质干细胞移植组穿越平台次数及目标象限游泳距离与总距离百分比均高于脑损伤组和骨髓间充质干细胞移植组（P＜0.05）。芬戈莫德＋骨髓间充质干细胞移植组 PKH-26阳性细胞明显高于脑损伤组和骨髓间充质干细胞移植组（P＜0.05）。结果可见骨髓间充质干细胞移植可明显改善重型颅脑损伤后大鼠的记忆功能，联合应用芬戈莫德免疫抑制剂有协同效果。

Changes in memory function of rats with brain injury after fingolimod administration combined with bone marrow mesenchymal stem cell transplantation

The repair effects of bone marrow mesenchymal stem celltransplantation on brain injury were not ideal. Combined therapy with medicine and biological engineering materials is needed. OBJECTIVE：To investigate the effects of bone marrow mesenchymal stem celltransplantation and fingolimod immunosuppressants on memory function recovery in rats with brain injury. METHODS：A total of 60 healthy Sprague-Dawley rats were subjected to hydraulic shock with peak value of 253.312 5-303.975 kPa with a hydraulic head injury instrument so as to induce a model of severe hydraulic head injury. They were randomly divided into brain injury group, bone marrow mesenchymal stem celltransplantation group and fingolimod＋bone marrow mesenchymal stem celltransplantation group. The Morris water maze test was tested at 21-28 days after PKH-26-labeled bone marrow mesenchymal stem celltransplantation. The PKH-26 immunofluorescence and hematoxylin-eosin staining were conducted in brain tissues at 4 weeks after brain injury. RESULTS AND CONCLUSION：At 4 weeks after transplantation, the average escape latency was gradual y decreased in each group. The average escape latency was shorter in the fingolimod＋bone marrow mesenchymal stem celltransplantation group than in the bone marrow mesenchymal stem celltransplantation group （P〈0.05）, and significantly shorter than in the brain injury group （P〈0.01）. The number of times of crossing the platform and the percentage of swimming distance to total distance were higher in the fingolimod＋bone marrow mesenchymal stem celltransplantation group than in the brain injury group and bone marrow mesenchymal stem celltransplantation group （P〈0.05）. The number of PKH-26-positive cells was significantly higher in the fingolimod＋bone marrow mesenchymal stem celltransplantation group than in the brain injury group and bone marrow mesenchymal stem celltransplantation group （P〈0.05）. Results confirmed that bone marrow mesenchymal stem celltransplantation could apparently improve memor