基因枪介导的骨形态发生蛋白2基因转染治疗陈旧性骨缺损

背景：体内外研究都已证实骨形态发生蛋白具有调节成骨细胞和成软骨细胞的分化、诱导异位骨形成、促进骨折愈合、控制哺乳动物骨骼不同形态特征形成的功能。目的：使用含有骨形态发生蛋白2基因真核表达质粒的基因枪进行局部基因注射以治疗陈旧骨缺损。方法：72只新西兰大白兔建立陈旧兔桡骨中段骨缺损模型，按所截骨长度均分1.5 cm组、2.0 cm和2.5 cm组。各组又随机分为治疗组（骨形态发生蛋白2基因转染组）和对照组（自然愈合组）。于转染后1，3，8，9周拍摄X射线平片，1，3，8和9周取骨折间软组织行骨形态发生蛋白2的Western blot检测，于1，3，8和9周时取标本大体观察，评价愈合情况。结果与结论：①大体标本观察发现治疗组骨痂生成量多于对照组。②治疗组转染后1，3，8，9周的Lane-Sandhu X射线评分优于对照组，差异均有显著性意义（P＜0.05）。③骨形态发生蛋白2浓度定量，各时间点治疗组骨形态发生蛋白2浓度优于对照组，差异均有显著性意义（P＜0.05）。结果说明应用基因枪介导骨形态发生蛋白2基因局部转移治疗陈旧骨缺损效果明确。

Particle gun-mediated bone morphogenetic protein-2 gene transfection for treatment of chronic bone defects

BACKGROUND：Both in vitro and in vivo studies have confirmed that, bone morphogenetic protein （BMP） regulates the differentiation of osteoblasts and chondroblasts, induces heterotopic bone formation, promotes fracture healing, and controls the morphology of skeleton in mammals.OBJECTIVE：To treat chronic bone defects using particle gun containing BMP2 gene eukaryotic expression plasmid via local injection.METHODS：A total of 72 healthy New Zealand white rabbits were applied to establish chronic bone defect model in the rabbit radius. According to the length of bone defect, the rabbits were divided into three groups：1.5 cm group, 2.0 cm group, 2.5 cm group. Each group was further randomly assigned into two subgroups：treatment group （BMP-2 gene transfection） and control group （natural y healing）. X-ray examinations were performed at 1, 3, 8 and 9 weeks after transfection, and soft tissue between the bone defects was harvested to detect BMP-2 using western blot analysis;and radius specimens were taken for gross observation at the same time points, to evaluate the healing.RESULTS AND CONCLUSION：（1） Gross specimen observation：bone cal us formation in treatment group was general y more than that in control group. （2） Lane-Sandhu X-ray score in treatment group was significantly＆amp;nbsp;higher than that in control group at 1, 3, 8, 9 weeks after transfection （P〈0.05）. （3） BMP-2 concentration in treatment group was significantly higher than that in control group at each time point （P〈0.05）. The local transfer of particle gun-mediated BMP-2 gene is an effective therapy of chronic bone defect.