高脂饮食肥胖模型小鼠脂肪组织受体相互作用蛋白140基因的表达

背景：受体相互作用蛋白140基因敲除小鼠可通过增加线粒体生物功能、脂肪酸氧化、氧化磷酸化等代谢途径来抵抗高脂饮食诱导的肥胖。 目的：构建高脂饮食致肥胖模型小鼠，观察脂肪组织受体相互作用蛋白140 mRNA表达水平变化及胰岛素抵抗的关系。 方法：将C57BL/6J雄性小鼠随机分为对照组和高脂饮食组，分别喂养14周后，测量2组小鼠体质量，选取高脂饮食组中体质量大于对照组小鼠平均体质量20%的小鼠作为肥胖组小鼠。 结果与结论：高脂饮食组小鼠中有12只符合标准计入肥胖组。肥胖组小鼠三酰甘油、总胆固醇、空腹血糖、空腹胰岛素水平和胰岛素抵抗指数均明显高于对照组（P＜0.05或P＜0.01）；肥胖组小鼠脂肪组织中受体相互作用蛋白140 mRNA的表达高于对照组（P＜0.05）；且小鼠脂肪组织受体相互作用蛋白140 mRNA表达水平与三酰甘油水平、胰岛素抵抗指数呈正相关（r=0.526，P＜0.05；r=0.465，P＜0.05），而与总胆固醇、空腹血糖、空腹胰岛素水平无相关性（P＞0.05）。

Receptor-interacting protein 140 mRNA expression in adipose tissue of a mouse model of high fat diet

BACKGROUND：Receptor-interacting protein 140-knockout mice are lean and resistant to high fat diet-induced obesity due to an increase in mitochondrial biogenesis, fatty acid oxidation and oxidative phosphorylation. OBJECTIVE：To study the expression level of receptor-interacting protein 140 mRNA in adipose tissue of high fat diet-induced obese mice and the correlation with insulin resistance. METHODS：Male C57BL/6J mice were randomly divided into control group and high fat diet group, and fed for 14 weeks separately. The mice of the two groups were weighed. Mice in high fat diet group whose body weight were 20%higher than average weight of control mice were selected as obese mice. RESULTS AND CONCLUSION：A total of 12 mice in high fat diet group were recruited into the obesity group. The levels of triglycerides, total cholesterol, fast blood glucose and fast insulin in obesity group were significantly higher than those of control group （P〈0.05 or P〈0.01）. The expression of receptor-interacting protein 140 mRNA in adipose was significantly higher in obesity group compared with the controls （P〈0.05）. Moreover, the expression of receptor-interacting protein 140 mRNA in adipose was positively correlated with triglycerides levels and insulin resistance index （r=0.526, P〈0.05;r=0.465, P〈0.05）, but negatively associated with the levels of total cholesterol, fast blood glucose and fast insulin （P〉0.05）.