人结直肠癌趋化因子配体生物轴及干细胞标志物基因表达与临床病理

背景：趋化因子配体12/趋化因子受体4生物学轴在肿瘤的特异性转移中有重要作用，而干细胞标志物糖蛋白激素受体5基因的表达对于肿瘤的增殖和侵袭转移发挥重要作用。 目的：观察趋化因子配体12/趋化因子受体4生物轴以及干细胞标志物糖蛋白激素受体5基因在人结直肠癌组织中的表达变化及其与临床中的病理特征的关系。 方法：收集2013年1至6月辽宁省肿瘤医院收治100名结直肠癌患者为实验组，100名健康体检者为对照组，采用免疫组织化学SP法检测两组组织中趋化因子配体12、趋化因子受体4及干细胞标志物糖蛋白激素受体5 mRNA表达情况，并分析趋化因子配体12、趋化因子受体4及干细胞标志物糖蛋白激素受体5 mRNA表达与结直肠癌患者年龄、性别、肿瘤大小及部位、淋巴转移以及预后等临床病理特征的相关性。 结果与结论：趋化因子受体4、糖蛋白激素受体5 mRNA在结直肠癌组织中均有较高的表达率，但是趋化因子配体12 mRNA表达率降低。趋化因子受体4、干细胞标志物糖蛋白激素受体5 mRNA、趋化因子配体12 mRNA三者与结直肠癌患者的年龄、性别等患者临床特征无相关性，与结直肠癌的发病位置及其大小也无相关性，与结直肠癌组织是否淋巴转移具有相关性关，伴有淋巴转移的结直肠癌组织中干细胞标志物糖蛋白激素受体5 mRNA和趋化因子受体4的表达率更高，而趋化因子配体12 mRNA表达无显著变化；趋化因子受体4表达随肿瘤的恶性程度增高而增高；糖蛋白激素受体5表达于胃肠道肿瘤和脑肿瘤干细胞等表面，其表达随肿瘤的恶性程度增高而增高。提示结直肠癌组织中趋化因子受体4的表达增高，糖蛋白激素受体5基因表达增高，二者增高促进了结直肠癌组织生长及转移，糖蛋白激素受体5以及趋化因子配体12/趋化因子受体4轴的表达的调控，使其或将成为肿瘤诊断及治疗的重要新靶点。

Biological axis of CXC chemokine ligand and expression of stem cell markers in human colorectal cancer tissues as well as their relationship with clinical and pathological characteristics

BACKGROUND：The biological axis of CXC chemokine ligand 12 （CXCL12）/CXC chemokine receptor 4 （CXCR4） plays an important role in the specific metastasis of tumors, whereas the mRNA expression of glycoprotein hormone receptor 5, a stem cellmarker, exerts an important effects on tumor proliferation, invasion and metastasis.
OBJECTIVE：To investigate the expressions of CXCL12/CXCR4 and glycoprotein hormone receptor 5 in human colorectal cancer tissues as wel as their relationship with clinical and pathological characteristics.
METHODS：Totaly 100 colorectal cancer patients admitted at Liaoning Cancer Hospital ＆ Institute from January to June 2013 were enroled as experimental group, and another 100 healthy volunteers served as control group. Immunohistochemical SP method was used to detect the expression of CXCL12, CXCR4 and glycoprotein hormone receptor 5 mRNA in human colorectal cancer tissues. We analyzed the correlation of CXCL12, CXCR4 and glycoprotein hormone receptor 5 mRNA expression with colorectal cancer patients’ age, sex, tumor size and site, lymphatic metastasis and prognosis .
RESULTS AND CONCLUSION：There was a higher expression of CXCR4 and glycoprotein hormone receptor 5 mRNA, but lower expression of CXCL12 mRNA in the human colorectal cancer tissues. The mRNA expression of CXCR4, CXCL12 and glycoprotein hormone receptor 5 showed no correlation with age, gender, tumor size and site of colorectal cancer patients, but was related to lymphatic metastasis. For colorectal cancer patients with lymphatic metastasis, the expression of CXCR4 and glycoprotein hormone receptor 5 mRNA was higher, but no change was found in the expression of CXCL12 mRNA. The expression of CXCR4 was increased with the degree of tumor malignancy as wel as glycoprotein hormone receptor 5 expressed on the surface of gastrointestinal cancer and brain tumor stem cels. These findings indicate that the growth and metastasis of human colorectal cancer tissues are promoted by increasing the expression of CXCR4 and glycoprotein hormone receptor 5 in human colorectal cancer tissues; the CXCL12/CXCR4 biological axis and glycoprotein hormone receptor 5 are expected to become a new target therapy for tumor diagnosis and treatment.