蛛网膜下腔阻滞不影响脐血单个核细胞向神经细胞的分化

背景：有学者认为麻醉状态可提高脐血采集分离质量，目前尚无蛛网膜下腔阻滞对脐血单个核细胞向神经干细胞及星形胶质细胞分化影响的研究。目的：观察蛛网膜下腔阻滞对新生儿脐血单个核细胞向神经干细胞及星形胶质细胞方向分化的影响。方法：选择顺产新生儿及蛛网膜下腔阻滞剖宫产新生儿脐血标本各20份，分别列为对照组及研究组，在胎儿娩出后抽取脐血分离接种脐血单个核细胞，并进行体外培养，每份标本常规培养3 d后分别进行非诱导分化培养和向神经细胞方向诱导分化培养。对培养前后细胞进行神经干细胞、星形胶质细胞标志性Nestin、GFAP抗原免疫组化鉴定。结果与结论：蛛网膜下腔阻滞剖宫产新生儿脐血单个核细胞诱导分化培养后表达 Nestin、GFAP 抗原阳性细胞，与顺产新生儿比较差异无显著性意义（P 〉0.05）。结果显示蛛网膜下腔阻滞对新生儿脐血单个核细胞向神经干细胞及星形胶质细胞方向分化没有影响。

Subarachnoid block has no influence on neural differentiation potential of mononuclear cells in the cord blood

BACKGROUND：Some scholars believe that anesthesia can improve the quality of cord blood separation and colection, and subarachnoid block is commonly used in cesarean section, but there is no research on the influence of subarachnoid block on differentiation potential of mononuclear cels into neural stem cels and astrocytes.
OBJECTIVE： To study the influence of subarachnoid block on differentiation potential of mononuclear cels in the cord blood of neonates into neural stem cels and astrocytes.
METHODS：Mononuclear cels isolated from 20 neonates delivered spontaneously and 20 neonates born by cesarean delivery undergoing subarachnoid block were culturedin vitro, acting as control group and study group. After delivery, cord blood samples were taken to isolate and culture mononuclear celsin vitro. After 3 days of routine culture, the cels were subject to non-induced culture and induced differentiation into neural stem cels. The cultured cels were identified by the cellmakers Nestin and glial fibrilary acidic protein with immunohistochemistry identification.
RESULTS AND CONCLUSION：The Nestin and glial fibrilary acidic protein showed no difference between the two groups after induced differentiation （P 〉 0.05）, indicating subarachnoid block has no impact on the differentiation of mononuclear cels in the cord blood from neonates into neural stem cels and astrocytes.