组织工程软骨与柚皮苷联合修复兔膝关节软骨缺损的组织学证实

背景：目前临床上虽有多种方法用于治疗软骨缺损，但没有从根本上解决关节软骨缺损修复问题。目的：通过组织学研究进一步评价柚皮苷结合组织工程软骨修复兔关节软骨缺损的效果。方法：取兔骨髓间充质干细胞体外增殖后，复合于改建后的脱细胞真皮基质载体上，制成组织工程软骨，植入到兔膝关节软骨缺损，并以柚皮苷汤灌胃，于4，8周后分别对修复组织进行苏木精-伊红、Masson三色染色、甲苯胺蓝染色、Ⅱ型胶原染色、Ⅹ型胶原染色等组织学检查。结果与结论：术后8周,柚皮苷结合干细胞复合体组缺损处修复组织变成乳白色，半透明光滑组织，缺损修复组织与周围正常软骨已基本难区分，表面光滑。组织学检查发现修复缺损处基本为新生软骨填充。结果证实，柚皮苷结合组织工程软骨能提高家兔膝关节软骨缺损的修复质量。

Histology research on repairing of rabbit articular cartilage defects with naringin and tissue engineering cartilage

BACKGROUND：At present, there are many methods to treat cartilage defects, but none radical y repairs the articular cartilage defects. OBJECTIVE：To histological y verify the effect of naringin combined with tissue engineering cartilage on the repair of rabbit articular cartilage defects. METHODS：Rabbit bone marrow mesenchymal stem cells fol owing in vitro proliferation were compounded onto acellular dermal matrix, which was then implanted into rabbit knee cartilage defects. Naringin was also given by lavage. Hematoxylin-eosin staining, Masson trichrome staining, toluidine blue dyeing, type II col agen staining and type X col agen staining were performed in the repaired tissue. RESULTS AND CONCLUSION：After 8 weeks post-surgery, the defects repaired with the naringin and stem cells composite were turned into milky-white and transparent smooth tissue. The defective tissue which was repaired, was very similar to normal cartilage tissue, with smooth surface. After the histology research, we found that the defect tissue was fil ed with new cartilage tissue. Results indicated that naringin combined with tissue engineering cartilage can promote the repair of articular cartilage defects in rabbits.