骨髓间充质干细胞移植修复高原大鼠股骨缺损

背景：对于高原地区受到环境负面影响的组织创伤，例如高原骨缺损，干细胞的特点可以提供一个良好的加速创伤修复的方法。目的：研究骨髓间充质干细胞治疗大鼠高原股骨缺损的创伤恢复效果。方法：分离提取雄性Wistar大鼠原代骨髓间充质干细胞，取第3代细胞进行细胞表型鉴定。雌性Wistar大鼠80只，制备实验性股骨圆形缺损后随机均分为平原对照组、平原骨髓间充质干细胞移植组、高原对照组、高原骨髓间充质干细胞移植组（n=20）。治疗后第30天拍X射线片观察股骨缺损区的修复情况，并于第10，20，30天分别采集股骨缺损区组织，检测其中碱性磷酸酶的含量。结果与结论：高原骨髓间充质干细胞移植组大鼠股骨缺损区愈合速度较高原对照组快，且碱性磷酸酶的含量较高原对照组高（P〈0．05）；平原骨髓间充质干细胞移植组的缺损区愈合速度也比平原对照组快，且碱性磷酸酶的含量也较平原对照组高（Pc0．05）。平原骨髓间充质干细胞移植组的缺损区愈合速度较高原骨髓间充质干细胞移植组快，且碱性磷酸酶的含量较高原骨髓间充质干细胞移植组高沪〈0．05）。说明骨髓间充质干细胞的局部移植可提高高原股骨缺损区局部的碱性磷酸酶含量，加速缺损区的创伤修复速度，但高原股骨缺损区的愈合修复较平原组慢。

Bone marrow mesenchymal stem cell transplantation for repair of rat femoral defects at high altitude

BACKGROUND： Stem cell transplantation can provide a good way to accelerate wound healing for tissue defects under negative environmental impacts at high altitude, such as plateau bone defects. OBJECTIVE： To research the therapeutic effects of bone marrow mesenchymal stem cell treatment on rat femoral defects in high altitude. METHODS： The bone marrow mesenchymal stem cells were separated from the femur and tibia of male Wistar rats, end the passage 3 cells were identified by detecting their surface antigen. Eighty female Wistar rats were operated for experimental femoral defects and randomly allocated into fours equal groups, namely, plain control group, plain transplantation group, plateau control group, and plateau transplantation group. Thirty days after treatment, rats were under X-ray to observe the repairment of femoral defects, and tissues from femoral defect area were sampled to detect the levels of alkaline phosphatase at 10, 20, 30 days, respectively. RESULTS AND CONCLUSION： The rats in the plateau transplantation group B showed significantly faster repair of femoral defect and higher level of alkaline phosphatase than those in the plateau control group （P 〈 0.05）. The rats in the plain control group also had better healing of femoral defect and higher level of alkaline phosphatase than those in the plain control group （P 〈 0.05）. Furthermore, the healing of femoral defect and level of alkaline phosphatase were better in the plain transplantation group than the plateau transplantation group. These findings indicate that intramuscular transplantation of bone marrow mesenchymal stem cells can significantly increase the regional content of alkaline phosphatase, promote the healing of femoral defects on the plateau, but the repairing speed of femoral defects in plateau is slower than that on the plain.