Balb/c 裸鼠体内的干细胞致瘤实验简

背景：干细胞致瘤性研究是临床应用干细胞安全性所面临的实际问题。因此明确干细胞是否具有致瘤性尤为重要。裸鼠在肿瘤学、免疫学、药品及生物制品的安全性评价领域占据愈来愈重要的地位。目的：将神经干细胞、间充质干细胞移植在Balb/c免疫缺陷裸鼠皮下，检测其是否具有致瘤性。方法：将免疫缺陷Balb/c裸鼠随机分为5组，除空白组外，阳性对照组、阴性对照组、神经干细胞组和间充质干细胞组分别将肝癌细胞HepG2、人视网膜色素上皮细胞、传代至第4代的神经干细胞和间充质干细胞接种于裸鼠皮下，于注射后12周进行解剖检查，观察裸鼠移植部位肿瘤形成情况。同时进行软琼脂克隆形成实验，考察神经干细胞和间充质干细胞在体外软琼脂克隆形成情况。结果与结论：裸鼠致瘤性实验结果显示，空白组、阴性对照组、神经干细胞组、间充质干细胞组在接种12周后，未在裸鼠注射部位形成肿瘤，病理组织学检查未见异常。软琼脂克隆形成实验结果显示，神经干细胞和间充质干细胞在软琼脂阻力介质中未表现出细胞形成克隆的能力。提示短期传代后的神经干细胞、间充质干细胞不具有致瘤性。

Stem cell tumorigenicity in Balblc nude mice

BACKGROUND：Stem celltumorigenicity is a practical issue concerning stability in the clinical application of stem cells. Therefore, it is particularly important to clear whether stem cells have tumorigenic ability or not. Nude mice occupy an increasingly important position in oncology, immunology, and safety evaluation of drugs and biological products. OBJECTIVE：To observe the tumorigenicity of neural stem cells and mesenchymal stem cells in Balb/c nude immunodeficient mice. METHODS：Balb/c nude mice were randomly divided into control group, negative group, positive group, neural stem cellgroup and mesenchymal stem cellgroup. HepG-2 cells, RPE cells, passage 4 neural stem cells and mesenchymal stem cells were injected subcutaneously into nude mice from different groups, respectively. After 12 weeks of injection, anatomical observation was performed to detect the tumor formation in the transplantation site. Meanwhile, soft agar colony formation assay was applied to investigate neural stem celland mesenchymal stem cellclone in vitro. RESULTS AND CONCLUSION：After 12 weeks of injection, the tumorigenicity study results showed that no tumor developed in the transplantation site in the control group, negative group, neural stem cellgroup and mesenchymal stem cellgroup. Histopathologic examinations also showed no abnormality in these groups. Soft agar colony formation assay showed in soft agar resistance medium, neural stem cells and mesenchymal stem cells did not exhibit clone ability. These findings indicate that neural stem cells and mesenchymal stem cells undergoing short-term passages have no tumorigenic growth.