大鼠原位异体肾移植硬化加快模型的建立

目的 建立大鼠移植肾慢性失功加快动物模型。方法 分别采用雄性SD大鼠和Wistar大鼠作为供受体，进行原位肾移植。术中强化缺血／再灌注损伤，观察术后2、4、6、8及12周各时相血肌酐及移植肾病理变化。结果 经强化缺血／再灌注损伤1h处理的异体原位肾移植大鼠于术后6周起血肌酐开始明显升高，8周出现明显的慢性同种异体移植肾肾病(CAN)的病理改变，与未强化缺血／再灌注损伤对照组相比有明显的统计学差异．结论采用SD→Wistar 大鼠作为供受体，通过强化缺血／再灌注损伤建立原位异体肾移植硬化加快模型简便可行。

A New Rat Model of Transplant Kidney-sclerosis Accelerated by Prolonged Cold Ischemia Time

OBJECTIVE: To establish an accelerated animal model of the chronic renal allograft dysfunction in rat. METHODS: Kidney transplantation was performed from SD to Wistar strain (allogeneic) according to the procedure of Kamada with some modification. Before the transplantation, the kidney was preserved in 0-4 degrees C heparin sodium chloride solution for prolonging the one hour. The serum creatinine level and pathological change of transplant kidney were observed in the 2nd, 4th, 6th, 8th and 12th weeks post-transplantation. RESULTS: After transplantation, the serum creatinine level of recipient rats obviously increased in the 6th week and the pathologic changes of chronic nephropathy evidently appeared in the 8th week when compared to those of the control group with non-reinforcement I/R injury; a statistically significant difference was noted. CONCLUSION: It is simple and feasible to establish a rat model of (SD-->Wistar) transplant kidney-sclerosis accelerated by prolonging I/R injury.