针灸对环磷酰胺化疗荷瘤小鼠血清白介素-7、白介素-18含量的影响

目的:观察针灸对环磷酰胺(CTX)化疗荷瘤小鼠血清白细胞介素-7(IL-7)和IL-18含量的影响,探讨针灸改善化疗所致免疫抑制,提高机体免疫功能的机制。方法:昆明小鼠64只随机分为空白组、模型组、针刺组、艾灸组,每组16只,采用种植S 180肉瘤,复制移植瘤模型,模型组、针刺组、艾灸组腹腔注射CTX,制备CTX荷瘤小鼠模型。针刺组、艾灸组分别针刺及艾灸"大椎"及双侧"膈俞""肾俞""足三里",均每日治疗1次,其中8只治疗3d,另外8只治疗5d。尾静脉取血计数外周血白细胞数,计算小鼠脾脏指数,ELISA法检测血清中IL-7和IL-18的含量。结果:注射CTX后,荷瘤小鼠免疫功能受损,脾脏指数下降,血清IL-18的含量以及白细胞数降低(P0.05)。治疗5d后,针刺组及艾灸组白细胞计数明显高于模型组(P0.05)。治疗3d后,艾灸组脾脏指数明显高于模型组(P0.05);治疗3d后,针刺组血清IL-7高于模型组,治疗5d后,仍高于模型组及艾灸组(P0.05);治疗3d和5d,针刺组血清IL-18均明显高于模型组及艾灸组(P0.05)。结论:提高血清中IL-7和IL-18的含量,促进多种细胞因子的产生和各种免疫细胞的成熟,从而增强机体细胞免疫和体液免疫功能,是针刺减轻烷化剂CTX对免疫功能造成的损伤,提高机体免疫功能的重要机制之一。

Effect of Acupuncture and Moxibustion Intervention on Contents of Serum Interleukin-7 and Interleukin-18 in Cyclophosphamide-induced Immune Suppression in Tumor-bearing Mice

Objective To observe the effect of acupuncture and moxibustion intervention on the contents of serum IL-7and IL-18 and white blood cell counts in cyclophosphamide(CTX)-induced hypofunction of immunity in tumor-bearing mice,so as to explore its mechanism underlying improvement of immune suppression caused by chemotherapy.Methods Sixty-four male Kunming mice were implanted with S 180 sarcoma at the left armpit(for making tumor-bearing model),and then,randomized into control group,model group,acupuncture group and moxibustion group(16mice in each group).The mice of the latter 3groups were injected with CTX(i.p.,150mg·kg-1·d-1),and those of the control group were injected(i.p.)with normal saline.Filiform acupuncture needle or moxibustion was applied to"Dazhui"(GV 14),unilateral"Geshu"(BL 17),"Shenshu"(BL 23)and"Zusanli"(ST 36)once daily for 3or 5days.Contents of serum IL-7and IL-18 were detected by ELISA.Results In comparison with the control group,the plasma leukocyte counts from day 2to day 5,the spleen index and serum IL-18 contents on day 3and5 after administration of CTX were significantly decreased,and serum IL-7contents on day 3and 5after CTX remarkably increased in the model group(P<0.05).Following the treatment,the spleen index on day 3and serum IL-18 content on day 5in the moxibustion group,serum IL-7and IL-18 contents on day 3and 5in the acupuncture group were significantly up-regulated(P<0.05).The effect of acupuncture was markedly superior to that of moxibustion in up-regulating serum IL-18 contents on day 3and 5after the treatment(P<0.05).No significant differences were found between the acupuncture and moxibustion groups in the leukocyte counts and spleen index after the treatment,and in serum IL-7contents on day 3(P>0.05).Conclusion Acupuncture and moxibustion can up-regulate the serum IL-7and IL-18 and white blood cell levels in immuno-suppression mice,which may contribute to their effects in relieving immune hypofunction caused by alkylating agent CTX.