电针对类风湿性关节炎大鼠血清及膝关节白介素-17和白介素-23的影响

目的:通过观察电针对类风湿性关节炎(RA)模型大鼠白介素-17(IL-17)和白介素-23(IL-23)表达的影响,探讨电针治疗RA的作用机制。方法:60只雄性Wistar大鼠,随机取8只为正常组,其余大鼠采用Ⅱ型胶原和弗氏不完全佐剂复制RA模型。将造模成功的24只大鼠随机分为模型组、泼尼松组和电针组,每组8只。电针针刺大鼠双侧的"足三里"和"昆仑"穴,强度为1~2mA,频率2Hz/100Hz,每次30min,每日1次,连续治疗10次。分别在造模前、造模后和治疗结束后测量大鼠的踝关节直径,分别采用酶联免疫吸附法、蛋白印迹法检测大鼠血清和膝关节内的IL-17和IL-23含量。结果:与正常组相比,模型组大鼠的踝关节直径、血清和膝关节内的IL-17和IL-23的含量均升高(P0.05);与模型组比较,经电针和泼尼松治疗后的大鼠踝关节直径、血清和膝关节内的IL-17和IL-23的含量均降低(P0.05);电针组与泼尼松组相比,除膝关节IL-17含量电针组高于泼尼松组外(P0.05),其余差异均无统计学意义(P0.05)。结论:电针对RA大鼠有治疗作用,减少血清和膝关节中IL-17和IL-23的分泌可能是其作用机制之一。

Effect of Electroacupuncture Intervention on Serum IL-17and IL-23Contents in Rheumatoid Arthritis Rats

Objective To observe the influence of electroacupuncture(EA)stimulation of"Zusanli"(ST 36)and"Kunlun"(BL 60)on serum and knee-joint IL-17 and IL-23 contents in rheumatoid arthritis(RA)rats so as to study its underlying mechanism in improving RA.Methods Male Wistar rats were randomly allocated to normal control,RA model,EA and Prednisolone groups(n=8in each group).The RA model was established by intra-planta injection of incomplete Freund's adjuvant and type Ⅱ collagen.EA(2Hz/100 Hz,1-2mA)was applied to bilateral"Zusanli"(ST 36)and "Kunlun"(BL 60)for 30 min,once daily for 10 days.The rats' ankle diameter was measured,and IL-17 and IL-23 contents in the serum and the knee-joint cavity were assayed by ELISA and Western blot,respectively.Results In comparison with the normal control group,the rats' ankle diameter,serum IL-17 and IL-23 contents and knee-joint IL-17 and IL-23 protein expression levels were significantly increased in the model group(P<0.05).After EA and Prednisolone treatment,compared with the model group,all the rats' ankle diameter,serum IL-17 and IL-23 contents and knee-joint IL-17 and IL-23 protein expression levels were decreased remarkably(P<0.05).No obvious differences were found between the EA and Prednisolone groups in the aforementioned indexes(P>0.05),except IL-17 protein expression level(being markedly lower in the Prednisolone group than in the EA group).Conclusion EA intervention can reduce inflammatory reaction of the ankle-joint in RA rats,which may be related to its effects in down-regulating serum and knee-joint IL-17 and IL-23 levels.