低频电针对多囊卵巢综合征大鼠糖代谢及氧化应激的影响

目的:观察低频电针对多囊卵巢综合征(PCOS)大鼠糖代谢和氧化应激的影响。方法:雌性SD大鼠随机分成模型组、电针组与对照组,每组10只。采用丙酸睾丸酮注射合并高脂饲料喂养复制PCOS模型。电针组电针"中脘""关元""三阴交"等穴,每次30min,每日1次,治疗5周。ELISA法检测血清睾酮(T)、性激素结合球蛋白(SHBG)、胰岛素(FINS),硫代巴比妥酸法和黄嘌呤氧化法检测血清丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性,并行口服糖耐量试验(OGTT)。计算稳态胰岛素指数(HOMA-IR)及新β细胞指数(MBCI)。HE染色观察卵巢组织结构。结果:治疗后电针组T和MDA含量较模型组显著降低(P0.05,P0.01);电针组SHBG含量和SOD活力在治疗后较模型组明显升高(P0.05,P0.01)。治疗后电针组FINS,餐后0.5h、1h、2h血糖及HOMA-IR较模型组明显降低(P0.01,P0.05),MBCI显著升高(P0.01)。模型组呈卵巢等囊性改变,黄体数目减少,卵泡内颗粒细胞层数较对照组增厚;电针组切片可见多个黄体存在,囊性卵泡减少,颗粒细胞层增厚。结论:不改变高脂饲料喂养的条件下行电针治疗,总体上调整了氧化应激状态,高胰岛素血症、胰岛素抵抗及胰岛素β细胞功能、胰岛素敏感性、糖耐量都能得到一定程度的恢复。

Effect of Low-frequency Electroacupuncture Intervention on Oxidative Stress and Glucose Metabolism in Rats with Polycystic Ovary Syndrome

Objective To observe the effect of low-frequency electroacupuncture(EA)treatment on oxidative stress and metabolism disorder in polycystic ovary syndrome(PCOS)rats,so as to study its underlying mechanisms in improving PCOS.Methods Thirty female SD rats were randomized into control group,model group and EA group(n=10in each group).The PCOS model was established by subcutaneous injection of testosterone propionate(1mg/100 g,10mg/mL)for 8weeks.EA(2Hz)was applied to"Zhongwan"(CV 12),"Guanyuan"(CV 4)and bilateral"Sanyinjiao"(SP 6)or"Housanli"(ST 36)for 30 min,once daily(except weekends)for 5weeks.Serum testosterone(T),sex hormone-binding globulin(SHBG)and fasting serum insulin(FINS)contents were detected by ELISA,serum malondialdehyde(MDA)and superoxide dismutase(SOD)levels were assayed by thiobarbituric acid(TBA)and xanthine oxidation(XTO)methods,respectively,and fasting blood glucose(FBG)was determined by roche glucose meter.In addition,homeostasis model assessment for insulin resistance(HOMA-IR)and modified B cell function index(MBCI)were calculated.Results Compared with the control group,serum T,FBG,FINS,HOMAIR,and serum MDA levels were significantly increased in the model group(P<0.01),while serum SHBG,MBCI and SOD levels were considerably decreased in the model group(P<0.01).Following EA treatment,all the increased serum T,FBG,FINS,HOMA-IR,and serum MDA levels,and the decreased serum SHBG,MBCI and SOD levels were reversed obviously(P<0.05,P<0.01).Conclusion EA treatment may normalize insulin sensitivity,ameliorate insulin resistance and hyperinsulinemia in PCOS rats,probably by regulating the function of pancreatic isletsβcell and by reducing oxidative stress and free androgen.