壮医药线点灸疗法对糖尿病性胃轻瘫大鼠胃窦Cajal间质细胞的影响

目的:观察壮医药线点灸对糖尿病性胃轻瘫(DGP)大鼠胃窦Cajal间质细胞(ICC)的影响,探讨壮医药线点灸治疗DGP的作用机制。方法:120只SD大鼠随机抽取空白组30只,其余90只在运用链脲佐菌素一次性腹腔注射法及高脂高糖饲料伴不规则饮食法制备DGP模型成功后随机抽取模型组与治疗组各30只。治疗组壮医药线点灸治疗,取"中脘""脾俞""胃俞""内关""后三里",每日治疗1次,5次为1个疗程,疗程间休息2d,连续3个疗程。治疗结束后测定各组大鼠的体质量、粪重;用炭粉混悬液灌胃法测定各组大鼠的胃肠推进率;免疫组化法检测胃窦酪氨酸激酶(c-kit)阳性ICC细胞率变化。结果:与空白组相比,模型组粪重显著升高,体质量、胃肠推进率、c-kit阳性细胞率明显降低(均P0.01);与模型组相比,治疗组粪重显著降低(P0.01),体质量、胃肠推进率、c-kit阳性细胞率明显提高(均P0.01)。结论:壮医药线点灸治疗能改善DGP大鼠胃ICC中c-kit的表达水平,恢复ICC正常的功能活动以提高胃肠推进率。

Effect of Moxibustion with Ignited Zhuang-medicine Medicated-thread on Interstitial Cells of Cajal in Gastric Antrum in Diabetic Gastroparesis Rats

Objective To observe the effect of Zhuang-medicine medicated-thread moxibustion therapy on interstitial cells of Cajal(ICC)in the gastric antrum of diabetic gastroparesis(DGP)rats,so as to investigate its mechanism underlying improving DGP.Methods Sprague-Dawley(SD)male rats were randomly divided into 3groups:control(n=30),model(n=30)and moxibustion(n=30).The DGP model was established by intraperitoneal injection of streptozotocin(STZ)and by feeding the rats with high fat-sugar forage.Zhuang-medicine medicated-thread moxibustion was applied to"Zhongwan"(CV 12),bilateral"Pishu"(BL 20),"Weishu"(BL 21),"Neiguan"(PC 6)and "Zusanli"(ST 36)once per day,for 3weeks except weekends.The gastrointestinal propulsion rate and weight of stool in 24 hwere determined,and c-kit(a marker for ICC)expression of the gastric antrum tissue was measured by immunohistochemistry.Results The stool weight was significantly higher in the model group than in the control group(P<0.01),but the rate of gastrointestinal propulsion and the rate of c-kit immunoreaction(IR)positive cells in the gastric antrum tissue were significantly lower in the model group than in the control group(P<0.01).After moxibustion,the increased stool weight and the decreased gastrointestinal propulsion rate and decreased c-kit IR-positive cell rate were reversed(P<0.01).Conclusion Zhuang-medicine medicated-thread moxibustion therapy can improve gastrointestinal function in DGP rats,which may be associated with its effect in up-regulating the expression of c-kit IR-positive ICC.