电针心包经穴对急性脑缺血大鼠血清及脑组织神经生长因子和神经生长抑制因子的影响

目的:探讨电针手厥阴心包经穴对急性脑缺血大鼠神经修复的作用机制。方法:将SD大鼠随机分为正常组、假手术组、模型组、心包经组、肺经组,每组10只。采用颈外动脉插入线栓法复制局灶性脑缺血模型。心包经组取"天泉""曲泽""内关""大陵"穴,肺经组取"天府""尺泽""列缺""太渊"穴,于造模后6、24、48、72h进行电针治疗,每次30min。用酶联免疫吸附法检测血清神经生长因子(NGF)和神经生长抑制因子(Nogo-A)含量,免疫组化法检测脑梗死区NGF和Nogo-A的表达。结果:与正常组、假手术组比较,模型组能上调NGF在血清中的含量及脑梗死区的表达(P0.01),心包经组、肺经组较模型组进一步上调(P0.01),心包经组提高NGF在血清的含量、脑梗死区表达的作用优于肺经组(P0.01)。与正常组、假手术组比较,模型组血清Nogo-A含量增多(P0.01),心包经组、肺经组少于模型组(P0.01),心包经组降低血清Nogo-A含量作用优于肺经组(P0.01);Nogo-A在脑梗死区的表达各组间差异无统计学意义(P0.05)。结论:电针可促进血清、脑组织中NGF的表达,并降低血清Nogo-A的含量,且心包经组作用优于肺经组,对急性脑缺血后神经修复具有一定促进作用。

Effect of Electroacupuncture Stimulation of Acupoints of the Pericardium Meridian on Serum NGF and Nogo-A Contents and Cerebral NGF and Nogo-A Expression in Cerebral Ischemia Rats

Objective To observe the effect of Electroacupuncture(EA)stimulation of"Tianquan"(PC 2),"Quze"(PC 3),"Neiguan"(PC 6),"Daling"(PC 7)of the Pericardium Meridian on cerebral angiogenesis in cerebral ischemia(CI)rats,so as to reveal its mechanisms underlying improvement of stroke.Methods A total of 50 SD rats were equally randomized into normal control,sham,model,EA-Pericardium-Meridian acupoints(EA-PCM)and EA-Lung-Meridian acupoint(EA-LUM)groups.The CI model was established by occlusion of the middle cerebral artery.EA(2-4V,20Hz)was applied to PC 2,PC 3,PC 6,PC 7and"Tianfu"(LU 3),"Chize"(LU 5),"Lieque"(LU 7),"Taiyuan"(LU 9)of the Lung Meridian for 30 min,once at timepoints of 0h,6h,24 h,48hand 72 h,respectively after modeling.Serum nerve growth factor(NGF)and Nogo protein-A(Nogo-A)contents were assayed by enzyme linked immunosorbent assay(ELISA),and cerebral NGF and Nogo-A immunoactivity levels in the ischemic cerebral tissue were detected by immunohistochemistry.Results(1)Compared to the normal control group,serum NGF and Nogo-A contents,and cerebral NGF immunoactivity level in the model group were significantly increased(P<0.01).Following EA interventions,serum and cerebral NGF levels were further significantly up-regulated in the EA-PCM and EA-LUM groups(P<0.01),while serum Nogo-A contents were down-regulated in the two EA groups(P<0.01).The effect of EA-PCM was markedly superior to that of EA-LUM in up-regulating serum and cerebral NGF levels and down-regulating serum Nogo-A level(P<0.01).No significant differences were found between the normal control and sham groups in serum and cerebral NGF and Nogo-A levels(P>0.05),and among the 5groups in cerebral Nogo-A levels(P>0.05).Conclusion EA stimulation of acupoints of both Pericardium Meridian and Lung Meridian can up-regulate serum NGF,cerebral NGF expression and downregulate serum Nogo-A in CI rats,and the effect of Pericardium Meridian is markedly superior to that of Lung Meridian,suggesting apossible better nerve repair effect of EA-PCM acupoints on ischemic brain.