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| 黄菊解毒片中盐酸小檗碱含量测定 | |
| 引用本文： | 蒋艳红,耿佳,赵丽红.黄菊解毒片中盐酸小檗碱含量测定[J].中国中医药信息杂志,2009,16(11):40-41. |
| 作者姓名： | 蒋艳红 耿佳 赵丽红 |
| 作者单位： | 1. 石家庄市华新制药厂,河北,石家庄,050091 2. 河北科技大学,河北,石家庄,050018 3. 河北省直属机关第二门诊部,河北,石家庄,050051 |
| 摘 要： | 目的建立黄菊解毒片中盐酸小檗碱含量的测定方法。方法采用HPLC法，以Diamonsil C18（4．6mm×250mm，5μm）为色谱柱；乙腈-0．033mol／L磷酸二氢钾溶液（30：70）为流动相；流速：1．0mL／min；检测波长：265nm。结果盐酸小檗碱在0．0432～0．864μg范围内样品含量与峰面积呈良好的线性关系；重复性RSD=0．89％（n=6）；平均回收率为99．64％，RSD=0．74％（n=5）。结论本方法稳定、可靠、灵敏度高、重复性好，可用于黄菊解毒片的质量控制。 |
| 关 键 词： | 黄菊解毒片 盐酸小檗碱 高效液相色谱法 含量测定 |
Content Determination of Berberine in Huangju Detoxication Tablets |
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| JIANG Yan-hong,GENG Jia,ZHAO Li-hong.Content Determination of Berberine in Huangju Detoxication Tablets[J].Chinese Journal of Information on Traditional Chinese Medicine,2009,16(11):40-41. | |
| Authors: | JIANG Yan-hong GENG Jia ZHAO Li-hong |
| Institution: | JIANG Yan-hong, GENG Jia, ZHAO Li-hong (1.Shijiazhuang Huaxin Pharmaceutical Factory, Shijiazhuang 050091, China： 2.Hebei University of Science and Technology, Shijiazhuang 050018, China; 3.The Second Outpatient Department Directly Under Government, Shijiazhuang 050051, China) |
| Abstract: | Objective To establish the method to determine the content of berberine in Huangju Detoxication Tablets. Methods HPLC was used with Diamonnsil C18 （4.6 mm × 250 mm, 5 μm） as column, acetonitrile-0.033 mol/L potassium dihydrogen phosphate （30 ： 70） as mobile phase, detection wavelength was at 265 nm, flow rate was 1.0 mL/min. Results In berberine range of 0.0432-0.864 μg, the escope and content of the sample peak area had a good linear relationship, reproducibility of RSD=0.89% （n =6）, and the average recovery rate was 99.64% and RSD=0.74% （n =5）. Conclusion This method is stable, reliable, with high sensitivity and good reproducibility, and can be used for quality control of Huangju Detoxication Tablets. |
| Keywords: | Huangju DetoxicationTablets berberine HPLC content determination |
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