| 骨髓基质细胞支持CD34+细胞扩增的实验研究 |
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| 引用本文： | 王吉刚,梁后杰,史曦凯,陈洁平,张翼军.骨髓基质细胞支持CD34+细胞扩增的实验研究[J].重庆医学,2003,32(8):1049-1050. |
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| 作者姓名： | 王吉刚 梁后杰 史曦凯 陈洁平 张翼军 |
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| 作者单位： | 第三军医大学西南医院肿瘤血液科,重庆,400038 |
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| 摘 要： | 目的体外模拟造血微环境，观察CD34^ 细胞在骨髓基质细胞支持下的扩增效应。方法将免疫磁珠阳性选择分选的骨髓CD34^ 细胞接种于构建的基质细胞层培养，通过骨髓基质细胞支持CD34^ 扩增的细胞总数及集落形成细胞数(Colonyforming Cell。CFC)的变化，评价骨髓基质细胞支持CD34^ 细胞扩增的功能。结果 扩增后细胞总数及CFC数分别增加，表示骨髓基质细胞能很好地支持CD34^ 细胞扩增。结论 体外证实了骨髓基质细胞在造血干细胞更新、增殖中具有重要作用。
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| 关 键 词： | 造血微环境 骨髓基质细胞 造血干细胞 |
| 文章编号： | 1671-8348(2003)08-1049-02 |
Experimental study on BMSCs to support CD34+ cell to proliferate |
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| WANG Ji gang,LIANG Hou jie,SHI Xi kai,et al..Experimental study on BMSCs to support CD34+ cell to proliferate[J].Chongqing Medical Journal,2003,32(8):1049-1050. |
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| Authors: | WANG Ji gang LIANG Hou jie SHI Xi kai |
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| Abstract: | Objective To observe the proliferation of CD34 + cells supported by bone marrow stromal cells through modeling hematopoietic microenvironment in vivo. Methods The function of bone marrow stromal cells(BMSCs) to support CD34 + cells selected by MACS to proliferate was evaluated through the numbers of cells and colony forming cell (CFC). Results The numbers of cells and colony forming cell (CFC) are increased sharply.Conclusion This study indicates that BMSCs play an important role in renew and proliferation of hematopoietic stem cells. |
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| Keywords: | hematopoietic microenvironment bone marrow stromal cells(BMSC) hematopoietic stem cells |
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