| 基因治疗骨质疏松、骨转移癌的可行性研究 |
| |
| 引用本文： | 刘继中,纪宗玲,胡蕴玉,陈苏民,陶惠人.基因治疗骨质疏松、骨转移癌的可行性研究[J].中国组织工程研究与临床康复,2001,5(21):54-55. |
| |
| 作者姓名： | 刘继中 纪宗玲 胡蕴玉 陈苏民 陶惠人 |
| |
| 作者单位： | 1. 解放军第四军医大学西京医院全军骨科研究所,
2. 解放军第四军医大学生物化学与分子生物学教研室, |
| |
| 摘 要： | 目的研究人骨保护素(OPG)转染小鼠胚胎成肌细胞C2C12后细胞形态及细胞周期的变化。方法采用Westernblot方法证实OPG在真核细胞中表达。对稳定转染OPG基因的C2C12细胞的形态、细胞周期等进行检测。结果成功克隆了人OPG编码区基因并构建了真核表达载体pcDNA3－OPG。结论人OPG编码区基因稳定转染的小鼠胚胎成肌细胞生长状态良好，DNA合成旺盛，无诱导细胞凋亡现象，为采用OPG基因治疗骨质疏松、骨转移癌等的可行性提供了依据。
|
| 关 键 词： | 骨保护素 细胞形态 细胞周期 |
| 文章编号： | 1007－5496(2001)11－0054－02 |
| 修稿时间： | 2001年7月30日 |
The feasible of gene therapy for osteoprosis and metastatic carcinoma of bone |
| |
| LIU Ji-zhong,JI Zong-ling,HU Yun-yu,et al..The feasible of gene therapy for osteoprosis and metastatic carcinoma of bone[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2001,5(21):54-55. |
| |
| Authors: | LIU Ji-zhong JI Zong-ling HU Yun-yu |
| |
| Abstract: | Objective To study the effect of human osteopro tegenin(OPG)on cell morphology and cell cycle of C2C12.Method OPG overexpression cell line C2C12was s elected and confirmed by Western blot.The cell morphologic analysis and cell cycle were determined by microscope and flow cytometer.Result The human OPG coding cDNA and it' s mammalian expression vector were obtained.Conclusion Transfection with OPG coding cDNA had no negative effect on target cells,all above suggested that gene therap y using OPG is of feasible methods in the future. |
| |
| Keywords: | osteoprotegenin cell morphology cell cycle |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
