| Effect of Bushenantai Recipe on the Expression of Endometrial LIF in Mice with Embryonic Implantation Dysfunction |
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| 引用本文： | 张明敏,黄玉琴,朱桂金,黄光英,董莉萍,张锦金. Effect of Bushenantai Recipe on the Expression of Endometrial LIF in Mice with Embryonic Implantation Dysfunction[J]. 华中科技大学学报(医学英德文版), 2008, 28(1): 65-68. DOI: 10.1007/s11596-008-0116-7 |
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| 作者姓名： | 张明敏 黄玉琴 朱桂金 黄光英 董莉萍 张锦金 |
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| 作者单位： | Department of Integrated Traditional Chinese and Western Medicine，Department of Integrated Traditional Chinese and Western Medicine，Center of Reproductive Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology，Department of Integrated Traditional Chinese and Western Medicine，Department of Integrated Traditional Chinese and Western Medicine，Department of Integrated Traditional Chinese and Western Medicine，Wuhan 430030 China |
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| 基金项目： | a grant from National Natu-ral Sciences Foundation of China (No. 30371829) |
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| 摘 要： | In order to observe the effect of Bushenantai recipe on the expression of endometrial leukemia-inhibitory factor （LIF） in mice with embryonic implantation dysfunction （EID）, 120 Kunming mice post coition were randomized into three groups： normal control group, model group and traditional Chinese medicine group （TCM group） （n=40 in each group）. Uterus was collected on the pregnancy day （Pd） 4, 5, 6 after an intravenous injection of Evan＇s blue. The endometrium was dyed by Evan＇s blue and the mean points of response were observed on Pd 5. The expression of LIF mRNA and protein was detected by RT-PCR and immunohistochemistry respectively and analyzed statistically by image system. The results showed that the number of implantation sites in model group was remarkably less than in normal control group and TCM group. There was no significant difference between normal control group and TCM group. The expression of LIF mRNA and protein in model group was delayed. Bushenantai recipe could increase the expression of LIF mRNA and protein in endometria of mice with EID. It was suggested that Bushenantai recipe could improve embryo implantation of mice with EID by promoting the endometrial LIF expression and endometrial decidualization.
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| 关 键 词： | 胚胎移植 白血病 造血功能 治疗方法 |
| 收稿时间： | 2007-01-21 |
Effect of Bushenantai recipe on the expression of endometrial LIF in mice with embryonic implantation dysfunction |
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| Mingmin Zhang,Yuqin Huang,Guijin Zhu,Guangying Huang,Liping Dong,Jinjin Zhang. Effect of Bushenantai recipe on the expression of endometrial LIF in mice with embryonic implantation dysfunction[J]. Journal of Huazhong University of Science and Technology. Medical sciences, 2008, 28(1): 65-68. DOI: 10.1007/s11596-008-0116-7 |
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| Authors: | Mingmin Zhang Yuqin Huang Guijin Zhu Guangying Huang Liping Dong Jinjin Zhang |
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| Affiliation: | 1. Department of Integrated Traditional Chinese and Western Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
2. Center of Reproductive Medicine, Tongfi Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | In order to observe the effect of Bushenantai recipe on the expression of endometrial leukemia-inhibitory factor (LIF) in mice with embryonic implantation dysfunction (EID), 120 Kun- ruing mice post coition were randomized into three groups: normal control group, model group and traditional Chinese medicine group (TCM group) (n=40 in each group). Uterus was collected on the pregnancy day (Pd) 4, 5, 6 after an intravenous injection of Evan's blue. The endometrium was dyed by Evan's blue and the mean points of response were observed on Pd 5. The expression of LIF mRNA and protein was detected by RT-PCR and immunohistochemistry respectively and analyzed statistically by image system. The results showed that the number of implantation sites in model group was remarkably less than in normal control group and TCM group. There was no significant difference between normal control group and TCM group. The expression of L1F mRNA and protein in model group was delayed. Bushenantai recipe could increase the expression of LIF mRNA and protein in endometria of mice with EID. It was suggested that Bushenantai recipe could improve em- bryo implantation of mice with EID by promoting the endometrial LIF expression and endometrial decidualization. |
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| Keywords: | Bushenantai recipe embryo implantation dysfunction leukemia-inhibitory factor |
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