树突状细胞以两种不同方式负载大肠癌细胞株体外刺激淋巴细胞抗肿瘤活性的比较

目的 比较树突状细胞(DC)以两种不同方式负载大肠癌细胞株体外刺激淋巴细胞的抗瘤活性.方法 分离正常人外周血单核细胞体外诱导DC,分别负载热休克诱导凋亡的大肠癌细胞株和肿瘤细胞裂解物,以此刺激淋巴细胞作为效应细胞,大肠癌细胞株为靶细胞.MTT法测定效应细胞对靶细胞的杀伤作用.结果 负载热休克大肠癌细胞的DC 与负载肿瘤细胞裂解物的DC都显示对靶细胞的杀伤活性,但前者的杀伤率明显高于后者(P＜0.05).结论 负载热休克肿瘤细胞的DC是一更为有效的负载方式.

Comparison of lymphocytic antitumor activity stimulated in vitro by dendritic cells loaded with colorectal cancer cell strain in two different ways

Objective To compare the lymphocytic antitumor activity stimulated in vitro by dendritic cells(DCs)loaded with colorectal cancer cell strain in two different wavs.Methods DCs isolated from peripheral blood mononuclear cell of healthy people were loaded with heat shock-induced apoptotlc colorectal cancer cell strain and tumor cell lysates,respectively.The lymphocytes stimulated in vitro by DCs were taken as effector cells and the colorectal cancer cell strain as target cells.Thc cellkilling effect of effector cells on target cells was determined by MTT method.Results Both of DCs loaded with heat shock-induced apoptotic colorectal cancer cell strain and tumor cell lvsates showed the killing activity on target cells,but the killing rate of the former was significantly higher than that of the latter(P＜0.05).Conclusion The method of loading with heat shock-indueed apoptotic colorectal cancer cell strain is more effective.