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| 海洋放线菌Streptomyces sp. SCSIO 1934中次生代谢产物的分离和鉴定 | |
| 引用本文： | 牛四文,李苏梅,田新朋,胡涛,鞠建华,杨晓红,张偲,张长生. 海洋放线菌Streptomyces sp. SCSIO 1934中次生代谢产物的分离和鉴定[J]. 中国中药杂志, 2011, 36(13): 1763-1768 |
| 作者姓名： | 牛四文 李苏梅 田新朋 胡涛 鞠建华 杨晓红 张偲 张长生 |
| 作者单位： | 1. 西南大学园艺园林学院,重庆,400715;中国科学院南海海洋研究所海洋生物资源可持续利用重点实验室海洋微生物研究中心广东省海洋药物重点实验室,广东广州,510301 2. 中国科学院南海海洋研究所海洋生物资源可持续利用重点实验室海洋微生物研究中心广东省海洋药物重点实验室,广东广州,510301 3. 西南大学园艺园林学院,重庆,400715 |
| 基金项目： | 中国科学院知识创新工程方向项目(KZCX2-EW-G-12,KSCX2-YW-G-065,KSCX2-YW-G-073);中国科学院百人计划项目(08SL111002);中国科学院南海海洋研究所青年基金项目(SQ200903)和领域方向性项目(LYQY200805);海洋生物资源可持续利用重点实验室开放基金项目(LMB091013);国家自然科学基金项目(41006089);广东省科技计划项目(20101303060010) |
| 摘 要： | 目的:从1株来源于中国南海沉积环境的海洋链霉菌SCSIO 1934的发酵产物中分离鉴定次生代谢产物.方法:对海洋链霉菌SCSIO 1934的发酵液进行有机溶剂萃取,利用硅胶、凝胶柱色谱等方法分离次生代谢产物,通过核磁数据和理化性质对各单体化合物进行结构鉴定.结果:从菌株Streptomyces sp.SCSIO 1934中分离纯化得到17-脱甲基格尔德霉素(17-O-demethylgeldanamycin,1),lebstatin(2),17-O-demethyllebstatin(3),尼日利亚菌素(nigericin,4),尼日利亚菌素钠盐(nigericin sodium salt,5),abierixin(6).结论:本研究发现了1株能够产生多种抗生素的海洋放线菌Streptomyces sp.SCSIO 1934. |
| 关 键 词： | 海洋放线菌 链霉菌 次生代谢产物 抗生素 南海 |
| 收稿时间： | 2010-11-02 |
Isolation and structural elucidation of secondary metabolites from marine Streptomyces sp. SCSIO 1934 |
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| NIU Siwen,LI Sumei,TIAN Xinpeng,HU Tao,JU Jianhu,YNAG Xiaohong,ZHANG Si and ZHANG Changsheng. Isolation and structural elucidation of secondary metabolites from marine Streptomyces sp. SCSIO 1934[J]. China Journal of Chinese Materia Medica, 2011, 36(13): 1763-1768 | |
| Authors: | NIU Siwen LI Sumei TIAN Xinpeng HU Tao JU Jianhu YNAG Xiaohong ZHANG Si ZHANG Changsheng |
| Affiliation: | College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China;CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China;CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China;CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;CAS Key Laboratory of Marine Bio-resources Sustainable Utilization, RNAM Center for Marine Microbiology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China |
| Abstract: | Marine Actinobacteria are emerging as new resources for bioactive natural products with promise in novel drug discovery. In recent years, the richness and diversity of marine Actinobacteria from the South China Sea and their ability in producing bioactive products have been investigated. The objective of this work is to isolate and identify bioactive secondary metabolites from a marine actinobacterium SCSIO 1934 derived from sediments of South China Sea. The strain was identified as a Streptomyces spieces by analyzing its 16S rDNA sequence. Streptomyces sp. SCSIO 1934 was fermented under optimized conditions and seven bioactive secondary metabolites were isolated and purified by chromatographic methods including colum chromatography over silica gel and Sephadex LH-20. Their structures were elucidated as 17-O-demethylgeldanamycin ( 1 ), lebstatin ( 2 ), 17-O-demethyllebstatin ( 3 ), nigericin ( 4 ), nigericin sodium salt ( 5 ), abierixin ( 6 ), respectively, by detailed NMR spectroscopic data (1H, 13C, COSY, HSQC and HMBC). This work provided a new marine actinobacterium Streptomyces sp. SCSIO 1934, capable of producing diverse bioactive natural products. |
| Keywords: | marine actinomycetes Streptomyces secondary metabolites antibiotics South China Sea |
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