电针不同穴位对大鼠子宫平滑肌电活动的影响

目的:比较电针不同穴区对子宫肌电活动的影响。方法:共79只Wistar大鼠,包括40只正常未孕和39只已孕18~19 d的临产大鼠,用1.5%氯醛糖(50 mg/kg)和25%乌拉坦(420 mg/kg)混合液腹腔麻醉。前者随机分为对照、内关、合谷、三阴交组,每组10只;后者随机分为对照、内关和三阴交组,每组13只。腹壁切开后,于左侧中段子宫浆膜下埋藏一对针式不锈钢电极,记录子宫平滑肌肌电活动。电针取双侧"内关"合谷"三阴交",刺激参数为:频率2/15 Hz,强度1~2 mA,持续20 min。结果:1)在正常非孕鼠上,与对照组比,电针"三阴交"后,子宫肌电爆发波的频率及幅度、慢波的幅度明显增加(P<0.05);电针"合谷"对爆发波的频率有类似"三阴交"的作用;而电针"内关"穴后,爆发波的频率及幅度和慢波的频率明显降低(P<0.05)。与内关组比较,"三阴交"合谷"电针期间和停电针后0-5 min大多数指标均有显著性差异(P<0.05,0.001)。2)在妊娠后期大鼠上,与对照组比,电针"三阴交"后,子宫肌电的爆发波频率和幅度及慢波的幅度增加(P<0.05)且持久;电针"内关"穴后,子宫肌电爆发波、慢波的频率明显减低(P<0.05);两组相比,爆发波的频率和幅度、慢波波幅电针期间和停针后许多时程均有显著性差异(P<0.05)。结论:电针"三阴交"和"合谷"穴可兴奋子宫平滑肌的电活动,"三阴交"穴的作用更强,而电针"内关"穴则抑制子宫肌的电活动,不同穴位的作用具有相对特异性。

Effect of Electroacupuncture of Different Acupoints on Myoelectrical Activities of the Uterus in Rats

Objective: To observe the effect of electroacupuncture(EA) of different acupoints on electrical activities of the uterus in rats.Methods: A total of 79 Wistar rats anesthetized with mixture solution of 1.5% chloralose(50 mg/kg) and 25% urethrane(420 mg/kg,i.p.).In the first part of this study,40 non-pregnant rats were randomized into control,"Neiguan"(PC 6),"Hegu"(LI 4) and "Sanyinjiao"(SP 6) groups,with 10 cases in each group.In the second part of this study,39 pregnant rats were evenly randomized into control,"Neiguan"(PC 6) and "Sanyinjiao"(SP 6) groups,with 13 cases in each group.Electrohysterogram was recorded by using a bipolar stainless steel electrode inserted in the sub-perimetrium layer of the mid part of the uterus.EA(2 mA,5/15 Hz) was respectively applied to bilateral PC6,LI4,and SP6 for 20 min.Results: 1) In non-pregnant rats,compared with control group,the amplitude and frequency of fast waves(burst action potentials) and the amplitude of slow waves in SP6 increased significantly(P<0.05),and EA of LI4 had a similar effect in increasing the frequency of fast waves;while following EA of PC6,both frequency and amplitude of fast waves and the frequency of slow waves decreased significantly(P<0.05).In comparison with PC6 group,most indexes of SP6 and LI4 groups during EA and 0-5 min after EA presented significant differences(P<0.05,0.001).2) In pregnant rats,compared with control group,both the amplitude and frequency of fast waves and the amplitude of slow waves of the electrohysterogram in SP6 group increased significantly(P<0.05) and lasted a longer!time;while the frequency of both fast and slow waves decreased considerably in PC6 group(P<0.05).Comparison between SP6 and PC6 groups showed significant differences in the frequency and amplitude of fast waves and the frequency of slow waves during EA and after EA(P<0.05).Conclusion: EA of SP6 and LI4 can activate the myoelectrical activities of the uterine in both non-pregnant and pregnant rats,and the effect of EA of SP6 is stronger,whereas EA of PC6 can inhibit the myoelectrical activities of the uterine.Different acupoints have their relatively specific effect.