共培养下成骨细胞与血管内皮细胞相互功能影响

目的:了解共培养条件下成骨细胞(ROB)与血管内皮细胞(RVEC)功能相互影响。方法:取传代兔成骨细胞与血管内皮细胞,首先将成骨细胞与血管内皮细胞以1∶0、1∶1、2∶1、4∶1比例联合直接共培养于培养皿中,另将成骨细胞与血管内皮细胞以0∶1、1∶1、2∶1、4∶1联合直接共培养于培养皿中的盖玻片上。通过对成骨细胞内碱性磷酸酶活性(ALP)测定及培养液中骨钙素(OC)定量测定,观察血管内皮细胞对成骨细胞功能的影响。通过血管内皮细胞计数、生长曲线测定,检测成骨细胞对血管内皮细胞的影响。结果:成骨细胞与血管内皮细胞比例为2∶1组碱性磷酸酶活性测定、骨钙素含量明显高于其他两实验组,但与对照组无明显区别。血管内皮细胞增殖能力高于其他两实验组,而与对照组无差异。结论:两种细胞在该培养体系中可以起到互相促进作用。

The interaction between osteoblasts and vascular endothelial cells co-culture in vitro

Objective: To explore the interaction between rabbit osteoblasts(ROB) and rabbit vascular endothelial cells(RVEC) co-culture in virto. Methods: Rabit osteoblasts and vascular endothelial cells were isolated and co-cultured in ratios of 1:0, 1:1, 2:1 and 4:1 within culture chambers. The two kinds of cells were also co-cultured in ratios of 0:1, 1:1, 2:1 and 4:1 on coverslip. The metabolic function of osteoblast was assessed by alkaline phosphatase (ALP ) activity and osteocalcin (OC) assay. Morphology of two kinds of cells and proliferation of vascular endothelial cells was observed.Results: The biologic compatibility was good between osteoblasts and vascular endothelial cells. The activity of ALP and OC in group with ratio of 2:1 was higher than the other two groups, but no difference was found compared with control group. The proliferation of RVEC was higher than the other two groups. Conclusions: Synergistic effect was demonstrated by co-culture of osteoblasts and vascular endothelial cells.