| 线粒体nad 1内含子2序列在石斛属植物分子鉴定中的应用 |
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| 引用本文： | 张婷 王峥涛 徐珞珊 周开亚. 线粒体nad 1内含子2序列在石斛属植物分子鉴定中的应用[J]. 医学教育探索, 2005, 0(7): 1059-1062 |
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| 作者姓名： | 张婷 王峥涛 徐珞珊 周开亚 |
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| 作者单位： | 中国药科大学生药学研究室 江苏南京210038(张婷，王峥涛，徐珞珊)，南京师范大学生命科学学院生物资源技术江苏省重点实验室 江苏南京210097(周开亚) |
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| 基金项目： | 国家自然科学基金资助项目(30171144) |
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| 摘 要： | 目的在兰科石斛属药用植物鉴定中应用新的分子标记。方法扩增并测定9种石斛属植物线粒体中NADH脱氢酶亚基1编码基因(nad 1)内含子2(in tron 2)的全长序列。结果比对后的nad 1 in tron 2序列长872bp,其中有17个变异位点,可以鉴别除粉花石斛D end robium lodd ig esii以外的8种植物。结论线粒体nad 1 in tron2序列可以作为一种新的分子标记用于石斛属植物的鉴定。
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| 关 键 词： | 石斛属 线粒体nad1intron2 分子标记 |
Application of mitochondrial nad 1 intron 2 sequences to molecular identification of some species of Dendrobium Sw. |
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| ZHANG Ting,WANG Zheng-tao. Application of mitochondrial nad 1 intron 2 sequences to molecular identification of some species of Dendrobium Sw.[J]. Researches in Medical Education, 2005, 0(7): 1059-1062 |
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| Authors: | ZHANG Ting WANG Zheng-tao |
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| Affiliation: | ZHANG Ting~,WANG Zheng-tao~ |
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| Abstract: | Objective Application of a new molecular marker to the identification of Dendrobium((Orchidaceae)) species.Methods Complete sequences of the mitochondrial nad 1 intron 2 for nine species of Dendrobium Sw.were amplified and determined.Results Seventeen variable sites were found in the aligned 872 bp of nad 1 intron 2 sequences.Eight of the nine Dendrobium species except D.loddigesii could be identified by the nad 1 intron 2 sequences.Conclusion The mitochondrial nad 1 intron 2 sequences could be used as a new molecular marker for the identification of Dendrobium species. |
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| Keywords: | Dendrobium Sw. mitochondrial nad 1 intron 2 molecular marker |
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